Quantification of colocalization of endocytosed or endogenous proteins in CD34+ MKs via the Pearson overlap coefficient
| Proteins . | Pearson coefficient . | N (number of cells analyzed) . | |
|---|---|---|---|
| A . | B . | ||
| Alexa568-scuPA | Alexa488-uPAT | 0.85 ± 0.03 | 7 |
| Alexa488-scuPA | Alexa568-FV | 0.53 ± 0.06 | 5 |
| Alexa488-uPAT | Alexa568-FV | 0.51 ± 0.12 | 5 |
| Alexa568-scuPA | Alexa488-fibrinogen | 0.70 ± 0.08 | 5 |
| Alexa488-scuPA | Alexa555-FcRAP | 0.65 ± 0.17 | 13 |
| scuPA | IFITM-3 | 0.66 ± 0.11 | 5 |
| scuPA | VWF | 0.38 ± 0.03 | 7 |
| uPAT | VWF | 0.38 ± 0.03 | 5 |
| Proteins . | Pearson coefficient . | N (number of cells analyzed) . | |
|---|---|---|---|
| A . | B . | ||
| Alexa568-scuPA | Alexa488-uPAT | 0.85 ± 0.03 | 7 |
| Alexa488-scuPA | Alexa568-FV | 0.53 ± 0.06 | 5 |
| Alexa488-uPAT | Alexa568-FV | 0.51 ± 0.12 | 5 |
| Alexa568-scuPA | Alexa488-fibrinogen | 0.70 ± 0.08 | 5 |
| Alexa488-scuPA | Alexa555-FcRAP | 0.65 ± 0.17 | 13 |
| scuPA | IFITM-3 | 0.66 ± 0.11 | 5 |
| scuPA | VWF | 0.38 ± 0.03 | 7 |
| uPAT | VWF | 0.38 ± 0.03 | 5 |