Analysis of experiments testing direct neutrophil presentation or crosspresentation of antigen, using human cells, in the absence of anti-CD3 or anti-CD28 antibodies
| First author . | Reference . | Antigen used . | T cells used . | Neutrophils used . | Results . |
|---|---|---|---|---|---|
| Fanger | 14 | Superantigen and tetanus toxoid | Tetanus-toxoid–specific CD4+ T cells expanded from a healthy donor | Stimulated for 24 h with IFN-γ and GM-CSF and then irradiated | T-cell proliferation in response to superantigen but not tetanus toxoid (protein or peptide) |
| Vono | 18 | CMV antigen pp65 and influenza virus HA | Primary CD4+ T cells from seropositive donors | Directly ex vivo (pulsed 1 hour with antigen) | Proliferation and cytokine production by T cells, which is inhibited by anti–HLA-DR antibodies |
| Meinderts | 23 | Tetanus toxoid | Directly ex vivo CD4+ and CD8+ T cells | Incubated ± opsonized red blood cells. Second population of neutrophils given to T cells on d 4 of culture | Proliferation and cytokine production of T cells. This was inhibited by anti-HLA-DR antibodies. Only CD4+ and not CD8+ T cells proliferated |
| Radsak | 25 | Superantigen Staphylococcus enterotoxin E and tetanus toxoid | Tetanus-toxoid–specific CD4+ T cell lines expanded from healthy donors | Treated for 24 h with IFN-γ and GM-CSF | Proliferation of T cells in response to superantigen and to tetanus toxoid, inhibited by anti–HLA-DR and anti-CD86 antibodies. Only CD4+ and not CD8+ cells proliferated. Only autologous and not heterologous neutrophils could stimulate proliferation. |
| Polak | 36 | Birch pollen antigen Bet v 1 | Bet v 1–specific CD4+ T cell lines derived from allergic patients | Stimulated with GM-CSF, IFN-γ, and IL-3 and then irradiated | Proliferation and cytokine production by T cells |
| Sharma | 37 | Soluble Leishmania antigen | Directly ex vivo total CD3+ T cells | Normal-density or low-density neutrophils, directly ex vivo | No proliferation |
| Iking-Konert | 33 | Elastase and tetanus toxoid | CD4+ T cell lines specific for elastase and tetanus toxoid | Treated for 24 h with IFN-γ and GM-CSF | Proliferation by T cells in response to autologous but not heterologous neutrophils with antigen |
| Davey | 45 | Tetanus toxoid and influenza virus M1 matrix protein, as well as superantigen | CD8+ T cell lines and primary peripheral blood mononuclear cells | Cultured for 48 h with Vγ9Vδ2 or MAIT cells, CD3/CD28 stimulation or HMB-PP to activate unconventional T cells or IFNγ, TNF, and/or GMCSF | T-cell proliferation and cytokine production in response to antigen processed and presented by neutrophils. CD8+ T-cell response to exogenous antigen (cross-presented antigen). Dependent on priming of the neutrophils by culture with unconventional T cells. |
| First author . | Reference . | Antigen used . | T cells used . | Neutrophils used . | Results . |
|---|---|---|---|---|---|
| Fanger | 14 | Superantigen and tetanus toxoid | Tetanus-toxoid–specific CD4+ T cells expanded from a healthy donor | Stimulated for 24 h with IFN-γ and GM-CSF and then irradiated | T-cell proliferation in response to superantigen but not tetanus toxoid (protein or peptide) |
| Vono | 18 | CMV antigen pp65 and influenza virus HA | Primary CD4+ T cells from seropositive donors | Directly ex vivo (pulsed 1 hour with antigen) | Proliferation and cytokine production by T cells, which is inhibited by anti–HLA-DR antibodies |
| Meinderts | 23 | Tetanus toxoid | Directly ex vivo CD4+ and CD8+ T cells | Incubated ± opsonized red blood cells. Second population of neutrophils given to T cells on d 4 of culture | Proliferation and cytokine production of T cells. This was inhibited by anti-HLA-DR antibodies. Only CD4+ and not CD8+ T cells proliferated |
| Radsak | 25 | Superantigen Staphylococcus enterotoxin E and tetanus toxoid | Tetanus-toxoid–specific CD4+ T cell lines expanded from healthy donors | Treated for 24 h with IFN-γ and GM-CSF | Proliferation of T cells in response to superantigen and to tetanus toxoid, inhibited by anti–HLA-DR and anti-CD86 antibodies. Only CD4+ and not CD8+ cells proliferated. Only autologous and not heterologous neutrophils could stimulate proliferation. |
| Polak | 36 | Birch pollen antigen Bet v 1 | Bet v 1–specific CD4+ T cell lines derived from allergic patients | Stimulated with GM-CSF, IFN-γ, and IL-3 and then irradiated | Proliferation and cytokine production by T cells |
| Sharma | 37 | Soluble Leishmania antigen | Directly ex vivo total CD3+ T cells | Normal-density or low-density neutrophils, directly ex vivo | No proliferation |
| Iking-Konert | 33 | Elastase and tetanus toxoid | CD4+ T cell lines specific for elastase and tetanus toxoid | Treated for 24 h with IFN-γ and GM-CSF | Proliferation by T cells in response to autologous but not heterologous neutrophils with antigen |
| Davey | 45 | Tetanus toxoid and influenza virus M1 matrix protein, as well as superantigen | CD8+ T cell lines and primary peripheral blood mononuclear cells | Cultured for 48 h with Vγ9Vδ2 or MAIT cells, CD3/CD28 stimulation or HMB-PP to activate unconventional T cells or IFNγ, TNF, and/or GMCSF | T-cell proliferation and cytokine production in response to antigen processed and presented by neutrophils. CD8+ T-cell response to exogenous antigen (cross-presented antigen). Dependent on priming of the neutrophils by culture with unconventional T cells. |
CMV, cytomegalovirus; HMB-PP, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate; IL-3, interleukin 3; TNF, tumor necrosis factor; MAIT, mucosa-associated invariant T cells; HA, hemagglutinin; HLA-DR, human leukocyte antigen, DR isotype.