In-gene IS mapped to the canine F8 gene using TES
| ID . | Sample . | Total IS . | F8 intron IS . | F8 exon IS . | True F8 IS (%) . | Undetermined F8 IS (%) . |
|---|---|---|---|---|---|---|
| ANG | 1 | 4 | 2 | 2 | 50.0 | 50.0 |
| ANG | 2 | 113 | 0 | 113 | 0 | 100.0 |
| ELI | 1 | 11 | 9 | 2 | 81.8 | 18.2 |
| ELI | 2 | 13 | 1 | 12 | 7.7 | 92.3 |
| VC | 1 | 63 | 22 | 41 | 34.9 | 65.1 |
| VEC | 2 | 12 | 5 | 7 | 41.7 | 58.3 |
| ALX | 1 | 5 | 1 | 4 | 20.0 | 80.0 |
| ALX | 2 | 10 | 2 | 8 | 20.0 | 80.0 |
| JUN | 1 | 18 | 6 | 12 | 33.3 | 66.6 |
| JUN | 2 | 42 | 9 | 33 | 21.4 | 78.6 |
| MZ | 1 | 5 | 2 | 3 | 40.0 | 60.0 |
| MZ | 2 | 12 | 6 | 6 | 50.0 | 50.0 |
| FLO | 1 | 13 | 9 | 4 | 69.2 | 30.8 |
| FLO | 2 | 26 | 21 | 5 | 80.8 | 19.2 |
| MG | 1 | 5 | 2 | 3 | 40.0 | 60.0 |
| MG | 2 | 0 | 0 | 0 | 0.0 | 0.0 |
| ID . | Sample . | Total IS . | F8 intron IS . | F8 exon IS . | True F8 IS (%) . | Undetermined F8 IS (%) . |
|---|---|---|---|---|---|---|
| ANG | 1 | 4 | 2 | 2 | 50.0 | 50.0 |
| ANG | 2 | 113 | 0 | 113 | 0 | 100.0 |
| ELI | 1 | 11 | 9 | 2 | 81.8 | 18.2 |
| ELI | 2 | 13 | 1 | 12 | 7.7 | 92.3 |
| VC | 1 | 63 | 22 | 41 | 34.9 | 65.1 |
| VEC | 2 | 12 | 5 | 7 | 41.7 | 58.3 |
| ALX | 1 | 5 | 1 | 4 | 20.0 | 80.0 |
| ALX | 2 | 10 | 2 | 8 | 20.0 | 80.0 |
| JUN | 1 | 18 | 6 | 12 | 33.3 | 66.6 |
| JUN | 2 | 42 | 9 | 33 | 21.4 | 78.6 |
| MZ | 1 | 5 | 2 | 3 | 40.0 | 60.0 |
| MZ | 2 | 12 | 6 | 6 | 50.0 | 50.0 |
| FLO | 1 | 13 | 9 | 4 | 69.2 | 30.8 |
| FLO | 2 | 26 | 21 | 5 | 80.8 | 19.2 |
| MG | 1 | 5 | 2 | 3 | 40.0 | 60.0 |
| MG | 2 | 0 | 0 | 0 | 0.0 | 0.0 |
IS mapped within a F8 intron were deemed as “true” IS as these regions are not represented in the AAV-cFVIII vector. IS mapped to a F8 exon were considered “undetermined,” as these could represent either true exonic insertions or V-V insertion events. Overall, 97/352 (27.6%) insertion sites were located in F8 introns.