coRAG2 treated cells were characterized for possible unintended genome-editing induced mutations at the site of genomic integration. Allele frequencies were normalized∗ to remove background signal (>0.2%), using a normalization factor of 0.96. Color coded bars match the legend in Figure 2F. To prevent re-cutting, the PAM sequence was changed from C∗C∗N to GGN. SNP: single nucleotide polymorphism; PAM: protospacer adjacent motif.