A panel of monoclonal and polyclonal antibodies specific to different portions of the HOD antigen and their characteristics
| No. . | Antibodies . | Specificity . | IgG subtype . | RBC binding . |
|---|---|---|---|---|
| 1 | Mouse pIgG anti–HEL | HEL | Polyclonal | +++ |
| 2 | 4B7 | HEL | IgG1 | ++ |
| 3 | 2F4 | HEL | IgG1 | ++ |
| 4 | GD7 | HEL | IgG2b | ++ |
| 5 | 4B7 + 2F4 | HEL | IgG1 blend | +++ |
| 6 | Rabbit pIgG anti–OVA | OVA | Polyclonal | +++++ |
| 7 | Mouse pIgG anti–OVA | OVA | Polyclonal∗ | ++ |
| 8 | MIMA29 anti-Duffy | Fy3 | IgG2a | +++++ |
| 9 | deMIMA29 | Fy3 | IgG2a | +++++ |
| 10 | CBC-512 anti-Duffy | Fy3 | IgG1 | +++++ |
| 11 | deCBC-512 | Fy3 | IgG1 | +++++ |
| No. . | Antibodies . | Specificity . | IgG subtype . | RBC binding . |
|---|---|---|---|---|
| 1 | Mouse pIgG anti–HEL | HEL | Polyclonal | +++ |
| 2 | 4B7 | HEL | IgG1 | ++ |
| 3 | 2F4 | HEL | IgG1 | ++ |
| 4 | GD7 | HEL | IgG2b | ++ |
| 5 | 4B7 + 2F4 | HEL | IgG1 blend | +++ |
| 6 | Rabbit pIgG anti–OVA | OVA | Polyclonal | +++++ |
| 7 | Mouse pIgG anti–OVA | OVA | Polyclonal∗ | ++ |
| 8 | MIMA29 anti-Duffy | Fy3 | IgG2a | +++++ |
| 9 | deMIMA29 | Fy3 | IgG2a | +++++ |
| 10 | CBC-512 anti-Duffy | Fy3 | IgG1 | +++++ |
| 11 | deCBC-512 | Fy3 | IgG1 | +++++ |
HOD-RBCs, RBCs expressing a recombinant antigen composed of HEL, OVA sequences comprising amino acids 251 to 349, and the complete Duffy b (Fyb) transmembrane protein.
RBC binding: The maximum binding level reached for each antibody by flow cytometry and summarized based on the median fluorescence intensity (MFI) values calculated: MFI 50 to 200 (+), MFI: 200 to 400 (++), MFI 400 to 1000 (+++), MFI 1000 to 2000 (++++), and MFI > 2000 (+++++). See supplemental Figure 3.
Primarily consisting of mouse IgG2a and IgG2b (supplemental Figure 2).