Table 1. CD20 variants identified by WES... | American Society of Hematology

Table 1.

CD20 variants identified by WES in pre-mosunetuzumab biopsy samples and in paired biopsy samples at progression

Variants before mosunetuzumab
Patient	Protein position and amino acid	Domain	Variant class	CD20 IHC	CD20⁺PAX5⁺ (%)	BOR
1	Q187H	EC	Missense	CD20⁺	81.6	CR
2	G53E	TM	Missense	CD20⁺	CD20⁺⁺	CR
3	L66fs	TM	Frameshift	CD20⁺	42.3	PD
	L66R	TM	Missense
	IL138-139fs	TM	Shift, truncating
4	M58K	TM	Missense	CD20⁺	67.2	PD
5	Y77N	EC	Missense	CD20⁺	95.1	PD
5	V82G	TM	Missense	CD20⁺	95.1	PD
6	A65P	TM	Missense	CD20⁺	44.3	CR
7	S121∗	TM	Stop	CD20⁺	80.6	SD
7	K175E	EC (epitope)	Missense	CD20⁺	80.6	SD
8	K142∗	TM	Stop	CD20⁺	93.2	PR

Variants before mosunetuzumab
Patient	Protein position and amino acid	Domain	Variant class	CD20 IHC	CD20⁺PAX5⁺ (%)	BOR
1	Q187H	EC	Missense	CD20⁺	81.6	CR
2	G53E	TM	Missense	CD20⁺	CD20⁺⁺	CR
3	L66fs	TM	Frameshift	CD20⁺	42.3	PD
	L66R	TM	Missense
	IL138-139fs	TM	Shift, truncating
4	M58K	TM	Missense	CD20⁺	67.2	PD
5	Y77N	EC	Missense	CD20⁺	95.1	PD
5	V82G	TM	Missense	CD20⁺	95.1	PD
6	A65P	TM	Missense	CD20⁺	44.3	CR
7	S121∗	TM	Stop	CD20⁺	80.6	SD
7	K175E	EC (epitope)	Missense	CD20⁺	80.6	SD
8	K142∗	TM	Stop	CD20⁺	93.2	PR

Variants at progression
Patient	Visit	Protein position and amino acid	Domain	Variant class	CD20 IHC	CD20⁺PAX5⁺ (%)	BOR
9	Pre	I80N	EC	Missense	CD20⁺	60.2	PD
	Post	I80N	EC	Missense	CD20⁺	29.6
		C167G	EC (epitope)	Missense	CD20⁺	29.6
10	Pre	WT			CD20⁺	65.2	PR
	Post	Q187∗	EC	Stop	CD20⁻	0.73
		A201fs	TM	Shift, truncating	CD20⁻	0.73
11	Pre	[192]-1spl	SS	Splice	CD20⁺	77.2	PD
11	Post	[192]-1spl	SS	Splice	CD20⁻	1.13	PD
12	Pre	WT			CD20⁺	81.3	PD
12	Post	[54]-1spl	SS	Splice	CD20⁻	0	PD
13	Pre	WT			CD20⁺	71.9	PR
13	Post	P160fs	EC	Shift, truncating	CD20⁻	0.02	PR

Variants at progression
Patient	Visit	Protein position and amino acid	Domain	Variant class	CD20 IHC	CD20⁺PAX5⁺ (%)	BOR
9	Pre	I80N	EC	Missense	CD20⁺	60.2	PD
	Post	I80N	EC	Missense	CD20⁺	29.6
		C167G	EC (epitope)	Missense	CD20⁺	29.6
10	Pre	WT			CD20⁺	65.2	PR
	Post	Q187∗	EC	Stop	CD20⁻	0.73
		A201fs	TM	Shift, truncating	CD20⁻	0.73
11	Pre	[192]-1spl	SS	Splice	CD20⁺	77.2	PD
11	Post	[192]-1spl	SS	Splice	CD20⁻	1.13	PD
12	Pre	WT			CD20⁺	81.3	PD
12	Post	[54]-1spl	SS	Splice	CD20⁻	0	PD
13	Pre	WT			CD20⁺	71.9	PR
13	Post	P160fs	EC	Shift, truncating	CD20⁻	0.02	PR

CD20⁺, ≥10% CD20⁺PAX5⁺ levels; CD20⁻, <10% CD20⁺PAX5⁺ levels; CD20⁺⁺, pathologist reviewed moderate to strong staining.

BOR, best overall response; EC, extracellular; fs, frameshift; PD, progressive disease; PR, partial response; SD, stable disease; SS, splice site; TM, transmembrane.

∗

indicates a translation stop codon.

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