Rate of FIXa inhibition by AT in purified and plasma-based assays
| Protein . | Chromogenic assay . | ELISA . | aPTT . |
|---|---|---|---|
| kobs (min−1 × 10−2) . | kobs (min−1 × 10−2) . | kobs (min−1 × 10−2) . | |
| FIXa-WT | 1.12 ± 0.2 | 1.45 ± 0.12 | 1.93 ± 0.17 |
| FIXa-V17I | 1.54 ± 0.4 | 1.79 ± 0.2 | 1.89 ± 0.08 |
| FIXa-V16L | ND | 0.119 ± 0.01 | 0.19± 0.02 |
| FIXa-V16T | ND | ND | ND |
| Protein . | Chromogenic assay . | ELISA . | aPTT . |
|---|---|---|---|
| kobs (min−1 × 10−2) . | kobs (min−1 × 10−2) . | kobs (min−1 × 10−2) . | |
| FIXa-WT | 1.12 ± 0.2 | 1.45 ± 0.12 | 1.93 ± 0.17 |
| FIXa-V17I | 1.54 ± 0.4 | 1.79 ± 0.2 | 1.89 ± 0.08 |
| FIXa-V16L | ND | 0.119 ± 0.01 | 0.19± 0.02 |
| FIXa-V16T | ND | ND | ND |
kobs values were determined by fitting the data in Figure 2A-C as detailed in “Methods.” The data are reported as the fitted values ± 2 SD. Data are representative of 3 independent experiments.
ND, not able to be determined.