Overview of selected candidate siRNAs with their target nucleotide(s)
| ID . | Target . | . | siRNA sequence . |
|---|---|---|---|
| siVwf | B6/129S | c.6348-c.6365 | 5'-dTAUGUUUUCACCaACGUACgsg-3' |
| siVwf.B6-1 | B6 | c.848G>A; c.852T>C | 5'-dTCGUUUACACCGcUGUUCCusc-3' |
| siVwf.B6-2 | B6 | c.1010T>C | 5'-dTGUACACAAAAUcUUCUCAcsa-3' |
| siVwf.B6-3 | B6 | c.1010T>C | 5'-dTCGUACACAAAAuCUUCUCasc-3' |
| siVwf.B6-4 | B6 | c.1010T>C | 5'-dTACGUACACAAAaUCUUCUcsa-3' |
| siVwf.B6-5 | B6 | c.2745T>C; c.2754A>G | 5'-dTGUACUCUGCACcCUGAUGgsa-3' |
| siVwf.B6-6 | B6 | c.2996G>A | 5'-dTUCACCGAGGGAuAGCUGCasa-3' |
| siVwf.B6-7 | B6 | c.2996G>A | 5'-dTUUCACCGAGGGaUAGCUGcsa-3' |
| siVwf.129S-1 | 129S | c.1089G>T; c.1090A>C | 5'-dTGACCUUCCUGGgCGCAGGusu-3' |
| siVwf.129S-2 | 129S | c.1089G>T; c.1090A>C | 5'-dTAGACCUUCCUGgGCGCAGgsu-3' |
| siVwf.129S-3 | 129S | c.2739C>T | 5'-dTAAACAGGGACAcCGCUCCasa-3' |
| siVwf.129S-4 | 129S | c.2745T>C; c.2754A>G | 5'-dTUACUCCGCACCcUGGUGGasa-3' |
| siVwf.129S-5 | 129S | c.2745T>C; c.2754A>G | 5'-dTGUACUCCGCACcCUGGUGgsa-3' |
| siVwf.129S-6 | 129S | c.2996G>A | 5'-dTGUUGCAACCCUcAUUUCCcsa-3' |
| siVwf.129S-7 | 129S | c.2996G>A | 5'-dTUUCACCGAGGGaUAGUUGcsa-3' |
| siControl | - | scrambled siVwf | 5'-dTGAUAUUUCCCUcAGUAACgsg-3' |
| siControl.B6 | - | scrambled siVwf.B6 | 5'-dTAUAUCCAAUUAcFCCUAAcsa-3' |
| siControl.129S | - | scrambled siVwf.129S | 5'-dTCGACAUCGAACgCGCACAasa-3' |
| ID . | Target . | . | siRNA sequence . |
|---|---|---|---|
| siVwf | B6/129S | c.6348-c.6365 | 5'-dTAUGUUUUCACCaACGUACgsg-3' |
| siVwf.B6-1 | B6 | c.848G>A; c.852T>C | 5'-dTCGUUUACACCGcUGUUCCusc-3' |
| siVwf.B6-2 | B6 | c.1010T>C | 5'-dTGUACACAAAAUcUUCUCAcsa-3' |
| siVwf.B6-3 | B6 | c.1010T>C | 5'-dTCGUACACAAAAuCUUCUCasc-3' |
| siVwf.B6-4 | B6 | c.1010T>C | 5'-dTACGUACACAAAaUCUUCUcsa-3' |
| siVwf.B6-5 | B6 | c.2745T>C; c.2754A>G | 5'-dTGUACUCUGCACcCUGAUGgsa-3' |
| siVwf.B6-6 | B6 | c.2996G>A | 5'-dTUCACCGAGGGAuAGCUGCasa-3' |
| siVwf.B6-7 | B6 | c.2996G>A | 5'-dTUUCACCGAGGGaUAGCUGcsa-3' |
| siVwf.129S-1 | 129S | c.1089G>T; c.1090A>C | 5'-dTGACCUUCCUGGgCGCAGGusu-3' |
| siVwf.129S-2 | 129S | c.1089G>T; c.1090A>C | 5'-dTAGACCUUCCUGgGCGCAGgsu-3' |
| siVwf.129S-3 | 129S | c.2739C>T | 5'-dTAAACAGGGACAcCGCUCCasa-3' |
| siVwf.129S-4 | 129S | c.2745T>C; c.2754A>G | 5'-dTUACUCCGCACCcUGGUGGasa-3' |
| siVwf.129S-5 | 129S | c.2745T>C; c.2754A>G | 5'-dTGUACUCCGCACcCUGGUGgsa-3' |
| siVwf.129S-6 | 129S | c.2996G>A | 5'-dTGUUGCAACCCUcAUUUCCcsa-3' |
| siVwf.129S-7 | 129S | c.2996G>A | 5'-dTUUCACCGAGGGaUAGUUGcsa-3' |
| siControl | - | scrambled siVwf | 5'-dTGAUAUUUCCCUcAGUAACgsg-3' |
| siControl.B6 | - | scrambled siVwf.B6 | 5'-dTAUAUCCAAUUAcFCCUAAcsa-3' |
| siControl.129S | - | scrambled siVwf.129S | 5'-dTCGACAUCGAACgCGCACAasa-3' |
Note: For both mouse strains, 7 strain-selective siRNAs were selected with the target nucleotide present in the B6 strain represented in bold and the corresponding nucleotide present in the 129S strain represented in roman font. siRNA sequences shown are of the antisense strands with the underscored nucleotides indicating the position of the nucleotide targets. Most siRNAs have only 1 nucleotide mismatch as target, whereas some have 2 mismatches. For the chosen in vivo candidate siRNAs, a corresponding scrambled siRNA was designed to act as a control. dT = DNA residue; G, C, A, U = RNA residue; g, c, a, u = 2’-O-methyl modified residue; s = phosphorothioate backbone modification.