Summary of all cell types used, their sources, and the respective analyses performed
| Experimental framework and components (X annotated indicates assay performed) . | |||||||
|---|---|---|---|---|---|---|---|
| No. . | Cell type . | Source . | MK culture . | ENKUR silencing . | ER stress induction . | RNA expression (MK and/or platelet) . | Protein expression (MK and/or platelet) . |
| 1 | Platelets | Peripheral blood (n = 49) | X CALR, CREB3L1, CDC20, and ENKUR | X CALR, CREB3L1, CDC20, and ENKUR | |||
| 2 | CD34+ | Bone marrow (n = 8) | X | X CALR, CREB3L1, CDC20, and ENKUR | |||
| 3 | CD34+ | Cord blood (n = 4) | X | X | X CALR, CREB3L1, CDC20, and ENKUR | X CALR, CREB3L1, CDC20, ENKUR, PI3K, and Akt | |
| 4 | CD34+ | Peripheral blood (n = 7) | X | X | X | X CALR, CREB3L1, CDC20, and ENKUR | X CALR, CREB3L1, CDC20, ENKUR, PI3K, and Akt |
| Experimental framework and components (X annotated indicates assay performed) . | |||||||
|---|---|---|---|---|---|---|---|
| No. . | Cell type . | Source . | MK culture . | ENKUR silencing . | ER stress induction . | RNA expression (MK and/or platelet) . | Protein expression (MK and/or platelet) . |
| 1 | Platelets | Peripheral blood (n = 49) | X CALR, CREB3L1, CDC20, and ENKUR | X CALR, CREB3L1, CDC20, and ENKUR | |||
| 2 | CD34+ | Bone marrow (n = 8) | X | X CALR, CREB3L1, CDC20, and ENKUR | |||
| 3 | CD34+ | Cord blood (n = 4) | X | X | X CALR, CREB3L1, CDC20, and ENKUR | X CALR, CREB3L1, CDC20, ENKUR, PI3K, and Akt | |
| 4 | CD34+ | Peripheral blood (n = 7) | X | X | X | X CALR, CREB3L1, CDC20, and ENKUR | X CALR, CREB3L1, CDC20, ENKUR, PI3K, and Akt |