Previously reported mice with Mecom mutation
| . | Mds1-Evi1 knockout . | Exon 3 deletion . | Exon 4 deletion . | Exon 7 deletion . | Junbo mice . | This study . |
|---|---|---|---|---|---|---|
| Reference | 41 | 37 | 38, 42 | 39, 44 | 40 | — |
| Genetic background | C57BL/6 | C57BL/6 | C57BL/6 | C57BL/6J | C3H/HeN | C57BL/6J |
| mEVI1 (XM_006535394.4) | Normal | 42-aa truncation at the N-terminus | Null | Null | c.2348A>T (p.N783I) | c.2255A>G (p.H752R) |
| mEVI1 Δ324 (NM_007963.2) | Normal | 42-aa truncation at the N-terminus | Null | Normal | c.1346A>T (p.N449I) | c.1253A>G (p.H418R) |
| mMDS1-EVI1 (NM_001361034.3) | Null | Null | Null | Null | c.2918A>T (p.N973I) | c.2825A>G (p.H942R) |
| Heterozygous mice | Mds1Δ/+ mice | Evi1Δ3/+ mice | Evi1Δ4/+ mice | Evi1Δ7/+ mice | Evi1KI/+ mice | |
| Homozygous mice | Mds1Δ/Δ mice | Evi1Δ3/Δ3 mice | Evi1Δ4/Δ4 mice | Evi1Δ7/Δ7 mice | Evi1KI/KI mice | |
| Lethality of homozygous mice | No (some mice die from malocclusion) | P0-3 | E13.5-16.5 | About E10.5 | E10.5 (17%) E18.5-birth (83%) | E10.5-11.5 |
| Hematologic abnormalities of mutant mice | (Homozygous) Reduced platelets and increased leukocytes in adult mice Reduced LSK cells and HSCs and increased GMPs in bone marrow | (Heterozygous) Reduced LSK and CD34+ LK cells in fetal livers | (Heterozygous) Reduced LSK and CD34+ LK cells in fetal livers Almost extinct long-term HSCs in bone marrow | (Heterozygous) Increased ratio of immature neutrophils to the total neutrophils | (Heterozygous) Reduced LSK cells in bone marrow from 8 to 12 weeks old Reduced platelets of adult mice |
| . | Mds1-Evi1 knockout . | Exon 3 deletion . | Exon 4 deletion . | Exon 7 deletion . | Junbo mice . | This study . |
|---|---|---|---|---|---|---|
| Reference | 41 | 37 | 38, 42 | 39, 44 | 40 | — |
| Genetic background | C57BL/6 | C57BL/6 | C57BL/6 | C57BL/6J | C3H/HeN | C57BL/6J |
| mEVI1 (XM_006535394.4) | Normal | 42-aa truncation at the N-terminus | Null | Null | c.2348A>T (p.N783I) | c.2255A>G (p.H752R) |
| mEVI1 Δ324 (NM_007963.2) | Normal | 42-aa truncation at the N-terminus | Null | Normal | c.1346A>T (p.N449I) | c.1253A>G (p.H418R) |
| mMDS1-EVI1 (NM_001361034.3) | Null | Null | Null | Null | c.2918A>T (p.N973I) | c.2825A>G (p.H942R) |
| Heterozygous mice | Mds1Δ/+ mice | Evi1Δ3/+ mice | Evi1Δ4/+ mice | Evi1Δ7/+ mice | Evi1KI/+ mice | |
| Homozygous mice | Mds1Δ/Δ mice | Evi1Δ3/Δ3 mice | Evi1Δ4/Δ4 mice | Evi1Δ7/Δ7 mice | Evi1KI/KI mice | |
| Lethality of homozygous mice | No (some mice die from malocclusion) | P0-3 | E13.5-16.5 | About E10.5 | E10.5 (17%) E18.5-birth (83%) | E10.5-11.5 |
| Hematologic abnormalities of mutant mice | (Homozygous) Reduced platelets and increased leukocytes in adult mice Reduced LSK cells and HSCs and increased GMPs in bone marrow | (Heterozygous) Reduced LSK and CD34+ LK cells in fetal livers | (Heterozygous) Reduced LSK and CD34+ LK cells in fetal livers Almost extinct long-term HSCs in bone marrow | (Heterozygous) Increased ratio of immature neutrophils to the total neutrophils | (Heterozygous) Reduced LSK cells in bone marrow from 8 to 12 weeks old Reduced platelets of adult mice |
aa, amino acid.