Cryo-EM data collection and processing and refinement statistics for C3bB-lufaxin (Mg2+) and C3bB-lufaxin-fXa (Mg2+)
| Parameters . | C3bB-lufaxin . | C3bB-lufaxin-fXa . |
|---|---|---|
| Accession numbers | PDB:8ENU, EMD: 28279 | PDB: 8EOK, EMD: 28378 |
| Data collection and processing | ||
| Magnification | 36 000× | 45 000× |
| Voltage (kV) | 200 | 200 |
| Total electron exposure/used (e/Å2) | 58.31 | 67.59 |
| Defocus range (μm) | −0.3 to −2.2 | −0.3 to −2.2 |
| Pixel size (Å2) | 0.56 | 0.46 |
| Processing program | Gautomatch, Relion 3.0.8 cisTEM, and EMAN2 | Gautomatch, Relion 3.2, and EMAN2 |
| Final particles used (number) | 80 727 | 248 281 |
| Symmetry imposed | C1 | C1 |
| Resolution (Å) (FSC threshold) | 3.22 | 3.53 |
| Refinement | ||
| Refinement program | Phenix | Phenix |
| Model composition | ||
| Nonhydrogen atoms | 20 118 | 22 275 |
| Protein residues | 2511 | 2798 |
| RMS deviations | ||
| Bond lengths (Å) | 0.003 | 0.002 |
| Bond angles (°) | 0.57 | 0.59 |
| Validation | ||
| MolProbity score | 1.74 | 1.78 |
| Clash score | 9.15 | 7.18 |
| Ramachandran plot (favored/allowed) | 96.2/100 | 94.7/100 |
| Mask CC | 0.76 | 0.81 |
| Parameters . | C3bB-lufaxin . | C3bB-lufaxin-fXa . |
|---|---|---|
| Accession numbers | PDB:8ENU, EMD: 28279 | PDB: 8EOK, EMD: 28378 |
| Data collection and processing | ||
| Magnification | 36 000× | 45 000× |
| Voltage (kV) | 200 | 200 |
| Total electron exposure/used (e/Å2) | 58.31 | 67.59 |
| Defocus range (μm) | −0.3 to −2.2 | −0.3 to −2.2 |
| Pixel size (Å2) | 0.56 | 0.46 |
| Processing program | Gautomatch, Relion 3.0.8 cisTEM, and EMAN2 | Gautomatch, Relion 3.2, and EMAN2 |
| Final particles used (number) | 80 727 | 248 281 |
| Symmetry imposed | C1 | C1 |
| Resolution (Å) (FSC threshold) | 3.22 | 3.53 |
| Refinement | ||
| Refinement program | Phenix | Phenix |
| Model composition | ||
| Nonhydrogen atoms | 20 118 | 22 275 |
| Protein residues | 2511 | 2798 |
| RMS deviations | ||
| Bond lengths (Å) | 0.003 | 0.002 |
| Bond angles (°) | 0.57 | 0.59 |
| Validation | ||
| MolProbity score | 1.74 | 1.78 |
| Clash score | 9.15 | 7.18 |
| Ramachandran plot (favored/allowed) | 96.2/100 | 94.7/100 |
| Mask CC | 0.76 | 0.81 |
CC, correlation coefficient; FSC, Fourier shell correlation; RMS, root mean squared.