List of unresolved issues in EV studies and possible solutions
| Issue . | Possible solution . |
|---|---|
| Lack of selective targets in exosome secretion | Differentiation by location. Unconventional exosome inhibitors such as proton pump inhibitors might work in the acidic BM109 Chemotherapy triggering the release of chemoexosomes might upregulate potential targets |
| Insufficient insight into the different secretion routes | Cargo profiling on the different routes by KO studies |
| Standardization of isolation techniques | Large consortium studies to compare different techniques at different sites to determine if cargo results are valid |
| Unsuitable isolation procedures for a clinical setting | Research into more feasible strategies without compromising exosome purity |
| Release of exosomes in the bloodstream, unassociated to MM, triggered by underlying pathologies such as chronic inflammation | For flow cytometry: always add an MM marker (CD38/CD138) For RNA analysis: an extra immunomagnetic isolation step, using an MM marker could be considered |
| Issue . | Possible solution . |
|---|---|
| Lack of selective targets in exosome secretion | Differentiation by location. Unconventional exosome inhibitors such as proton pump inhibitors might work in the acidic BM109 Chemotherapy triggering the release of chemoexosomes might upregulate potential targets |
| Insufficient insight into the different secretion routes | Cargo profiling on the different routes by KO studies |
| Standardization of isolation techniques | Large consortium studies to compare different techniques at different sites to determine if cargo results are valid |
| Unsuitable isolation procedures for a clinical setting | Research into more feasible strategies without compromising exosome purity |
| Release of exosomes in the bloodstream, unassociated to MM, triggered by underlying pathologies such as chronic inflammation | For flow cytometry: always add an MM marker (CD38/CD138) For RNA analysis: an extra immunomagnetic isolation step, using an MM marker could be considered |