Table 3. Antibodies and secondary... | American Society of Hematology

Table 3.

Antibodies and secondary reagents used for multiplex immunofluorescence panels

	Primary antibody	Primary antibody dilution	Clone	Manufacturer	Secondary antibody (Ventana)	TSA-conjugated fluorochrome
1	CD11c	1:200	5D11	Leica	Omnimap	Opal-520
2	CD30	1:25	BerH2	Cell Marque	Ultramap	Opal-540
3	CD68P∗	1:1500	PGM1	Dako	Omnimap	Opal-570
4	IDO1∗	1:4000	V1NC3IDO	Invitrogen	Omnimap	Opal-620
5	PD-L1∗	Predilute	SP263	Ventana	Optiview	Opal-650
6	TIM-3∗	1:200	D5D5R	Cell Signaling Technology	Omnimap	Opal-690
7	CADM1†	1:10,000	Polyclonal	Sigma-Aldrich	Omnimap	Opal-570
8	CD1C†	1:100	OTI2F4	Abcam	Omnimap	Opal-620
9	LAMP3†	1:100	1010E1	Dendritics	Ultramap	Opal-650
10	CD123†	1:200	BR4MS	Leica	Optiview	Opal-690

	Primary antibody	Primary antibody dilution	Clone	Manufacturer	Secondary antibody (Ventana)	TSA-conjugated fluorochrome
1	CD11c	1:200	5D11	Leica	Omnimap	Opal-520
2	CD30	1:25	BerH2	Cell Marque	Ultramap	Opal-540
3	CD68P∗	1:1500	PGM1	Dako	Omnimap	Opal-570
4	IDO1∗	1:4000	V1NC3IDO	Invitrogen	Omnimap	Opal-620
5	PD-L1∗	Predilute	SP263	Ventana	Optiview	Opal-650
6	TIM-3∗	1:200	D5D5R	Cell Signaling Technology	Omnimap	Opal-690
7	CADM1†	1:10,000	Polyclonal	Sigma-Aldrich	Omnimap	Opal-570
8	CD1C†	1:100	OTI2F4	Abcam	Omnimap	Opal-620
9	LAMP3†	1:100	1010E1	Dendritics	Ultramap	Opal-650
10	CD123†	1:200	BR4MS	Leica	Optiview	Opal-690

TSA, tyramide signal amplification.

CD11c and CD30 are used in both multiplexes. Other antibodies are annotated, as below:

Multiplex 1 only.

Multiplex 2 only.