Table 1. Rh phenotypes of iPSCs used to... | American Society of Hematology

Table 1.

Rh phenotypes of iPSCs used to generate iRBCs

Lines	PSC	ABO	RHD∗ RHCE∗ genotypes and presumed haplotypes	Rh antigen phenotype									Application as reagent RBC in antibody identification
Lines	PSC	ABO	RHD∗ RHCE∗ genotypes and presumed haplotypes	D	C	c	E	e	hr^S	hr^B	V	VS	Application as reagent RBC in antibody identification
DCe (R1R1)	ESC	O	∗D ∗Ce homozygous	+	+	0	0	+	+	+	0	0	Routinely used Rh phenotype lacking c and E
DcE (R2R2)	iPSC	O	∗D ∗cE No RHD ∗cE	+	0	+	+	0	0	+	0	0	Routinely used Rh phenotype lacking C and e
ce (rr)	iPSC	O	No RHD ∗ce homozygous	0	0	+	0	+	+	+	0	0	Routinely used Rh phenotype lacking D, C, and E
hr^S−	iPSC∗	O	∗DAU0 ∗ceMO ∗DOL ∗ceBI	+	0	+	0	+	0	+	0	0	Uncommon; lacks high-prevalence antigen hr^S Anti-hr^S would be (0) nonreactive
hr^B−	iPSC∗	O	∗DIIIa-CE(4-7)-D ∗ceS homozygous	+	+	+	0	+	+	0	0	0	Uncommon; lacks high-prevalence antigen hr^B Anti-hr^B would be (0) nonreactive
V⁺VS⁺	iPSC∗	O	∗D ∗ce733G ∗DFV ∗ce733G	+	0	+	0	+	+	+	+	+	Uncommon; expresses low-prevalence antigens V and VS Anti-V/VS would be (+) reactive
Rh null	iPSC	O	No RHD ∗ce (1kb del. KO) homozygous	0	0	0	0	0	0	0	0	0	Exceedingly rare; lacks RhD and RhCE proteins (0) nonreactive suggests antibody in the Rh system
D--	iPSC	O	RHD cDNA inserted at AAVS1 locus of Rh null iPSC	+	0	0	0	0	0	0	0	0	Exceedingly rare; expresses RhD and no RhCE (0) nonreactive suggests antibody to RhCE antigens
Go^a+	iPSC	O	RHD∗DIVa cDNA inserted at AAVS1 locus of Rh null iPSC	+	0	0	0	0	0	0	0	0	Expresses low-prevalence antigen Go^a, RhD, and no RhCE Anti-Go^a would be (+) reactive
DAK⁺	iPSC	O	RHD∗DIIIa cDNA inserted at AAVS1 locus of Rh null iPSC	+	0	0	0	0	0	0	0	0	Expresses low-prevalence antigen DAK, RhD, and no RhCE Anti-DAK would be (+) reactive

Lines	PSC	ABO	RHD∗ RHCE∗ genotypes and presumed haplotypes	Rh antigen phenotype									Application as reagent RBC in antibody identification
Lines	PSC	ABO	RHD∗ RHCE∗ genotypes and presumed haplotypes	D	C	c	E	e	hr^S	hr^B	V	VS	Application as reagent RBC in antibody identification
DCe (R1R1)	ESC	O	∗D ∗Ce homozygous	+	+	0	0	+	+	+	0	0	Routinely used Rh phenotype lacking c and E
DcE (R2R2)	iPSC	O	∗D ∗cE No RHD ∗cE	+	0	+	+	0	0	+	0	0	Routinely used Rh phenotype lacking C and e
ce (rr)	iPSC	O	No RHD ∗ce homozygous	0	0	+	0	+	+	+	0	0	Routinely used Rh phenotype lacking D, C, and E
hr^S−	iPSC∗	O	∗DAU0 ∗ceMO ∗DOL ∗ceBI	+	0	+	0	+	0	+	0	0	Uncommon; lacks high-prevalence antigen hr^S Anti-hr^S would be (0) nonreactive
hr^B−	iPSC∗	O	∗DIIIa-CE(4-7)-D ∗ceS homozygous	+	+	+	0	+	+	0	0	0	Uncommon; lacks high-prevalence antigen hr^B Anti-hr^B would be (0) nonreactive
V⁺VS⁺	iPSC∗	O	∗D ∗ce733G ∗DFV ∗ce733G	+	0	+	0	+	+	+	+	+	Uncommon; expresses low-prevalence antigens V and VS Anti-V/VS would be (+) reactive
Rh null	iPSC	O	No RHD ∗ce (1kb del. KO) homozygous	0	0	0	0	0	0	0	0	0	Exceedingly rare; lacks RhD and RhCE proteins (0) nonreactive suggests antibody in the Rh system
D--	iPSC	O	RHD cDNA inserted at AAVS1 locus of Rh null iPSC	+	0	0	0	0	0	0	0	0	Exceedingly rare; expresses RhD and no RhCE (0) nonreactive suggests antibody to RhCE antigens
Go^a+	iPSC	O	RHD∗DIVa cDNA inserted at AAVS1 locus of Rh null iPSC	+	0	0	0	0	0	0	0	0	Expresses low-prevalence antigen Go^a, RhD, and no RhCE Anti-Go^a would be (+) reactive
DAK⁺	iPSC	O	RHD∗DIIIa cDNA inserted at AAVS1 locus of Rh null iPSC	+	0	0	0	0	0	0	0	0	Expresses low-prevalence antigen DAK, RhD, and no RhCE Anti-DAK would be (+) reactive

cDNA, complementary DNA; +, predicted positive for the antigen; 0, negative for the antigen.

Indicates iPSCs reprogrammed from rare donors.