Table 1. Summary of classic serologic... | American Society of Hematology

Table 1.

Summary of classic serologic findings in AIHA

Type of AIHA	Antibody class	Antibody specificity	Optimum test phase of antibody reactivity	DAT result	Eluate result
Warm	IgG	Broadly reactive	IAT	2-4+ IgG and C3 or IgG only	Positive—reactive with all/majority of panel RBCs
Cold	IgM	I/i	RT, 37 °C^a	2-3+ C3 only	Not usually performed
Mixed Type	IgG and IgM	Broadly reactive	RT, 37 °C, IAT	3-4+ IgG and C3	Positive—reactive with all/majority of panel RBCs

Type of AIHA	Antibody class	Antibody specificity	Optimum test phase of antibody reactivity	DAT result	Eluate result
Warm	IgG	Broadly reactive	IAT	2-4+ IgG and C3 or IgG only	Positive—reactive with all/majority of panel RBCs
Cold	IgM	I/i	RT, 37 °C^a	2-3+ C3 only	Not usually performed
Mixed Type	IgG and IgM	Broadly reactive	RT, 37 °C, IAT	3-4+ IgG and C3	Positive—reactive with all/majority of panel RBCs

^a

results after 15-to-30 minute incubation at 37 °C.

2-4+, agglutination strength; RT, direct agglutination at room temperature.