Coagulant activity of FVII variants determined by a modified PT assay
| rFVII . | Clotting time ± SEM, s . | FVII activity, ng/mL . |
|---|---|---|
| WT | 18.60 ± 0.39 | 88.04 ± 2.92 |
| R147A | 48.82 ± 1.65 | 6.91 ± 0.93 |
| K192A | 58.22 ± 1.81 | 3.12 ± 0.45 |
| R147A/K192A | 95.13 ± 0.99 | ND |
| rFVII . | Clotting time ± SEM, s . | FVII activity, ng/mL . |
|---|---|---|
| WT | 18.60 ± 0.39 | 88.04 ± 2.92 |
| R147A | 48.82 ± 1.65 | 6.91 ± 0.93 |
| K192A | 58.22 ± 1.81 | 3.12 ± 0.45 |
| R147A/K192A | 95.13 ± 0.99 | ND |
After incubation of FVII variants (5 nM) with FVII-deficient plasma for 5 minutes, coagulant activity was determined using a modified PT assay. Clotting times were converted to FVII levels using a FVII standard curve. Data were expressed as mean ± SEM (n = 3).
ND, not detectable.