Primers used for generating the FVII mutations
| Mutation . | Primer . |
|---|---|
| R147A forward: | 5′- CAG CTG CTG GAC GCC GGC GCC ACG -3′ |
| R147A reverse: | 5′- CAG GGC CGT GGC GCC GGC GTC CAG -3′ |
| K192A forward: | 5′- G GAC TCC TGC GCC GGG GAC AGT GGA G -3′ |
| K192A reverse: | 5′- C TGT CCC CGG CGC AGG AGT CCT TGC TGC -3′ |
| Mutation . | Primer . |
|---|---|
| R147A forward: | 5′- CAG CTG CTG GAC GCC GGC GCC ACG -3′ |
| R147A reverse: | 5′- CAG GGC CGT GGC GCC GGC GTC CAG -3′ |
| K192A forward: | 5′- G GAC TCC TGC GCC GGG GAC AGT GGA G -3′ |
| K192A reverse: | 5′- C TGT CCC CGG CGC AGG AGT CCT TGC TGC -3′ |
Codon for mutated amino acid is in bold.