Mass spectrum analysis of SPAG6-interacting proteins
| Protein ID . | Protein Q score . | Protein mass (kDa) . | Unique peptide number . | Abundance . |
|---|---|---|---|---|
| sp|O94832|MYO1D | 129.88 | 116.13 | 36 | 1.34 × 108 |
| sp|Q69YQ0|CYTSA | 29.28 | 124.53 | 8 | 5.90 × 106 |
| sp|Q92922|SMRC1 | 18.62 | 122.79 | 6 | 1.12 × 107 |
| sp|O60763|USO1 | 17.84 | 107.83 | 5 | 1.23 × 108 |
| sp|Q9H3U1|UN45A | 13.34 | 103.01 | 4 | 2.22 × 106 |
| sp|O95235|KI20A | 11.83 | 100.22 | 3 | 7.90 × 105 |
| Protein ID . | Protein Q score . | Protein mass (kDa) . | Unique peptide number . | Abundance . |
|---|---|---|---|---|
| sp|O94832|MYO1D | 129.88 | 116.13 | 36 | 1.34 × 108 |
| sp|Q69YQ0|CYTSA | 29.28 | 124.53 | 8 | 5.90 × 106 |
| sp|Q92922|SMRC1 | 18.62 | 122.79 | 6 | 1.12 × 107 |
| sp|O60763|USO1 | 17.84 | 107.83 | 5 | 1.23 × 108 |
| sp|Q9H3U1|UN45A | 13.34 | 103.01 | 4 | 2.22 × 106 |
| sp|O95235|KI20A | 11.83 | 100.22 | 3 | 7.90 × 105 |
CYTSA, cytospin-A; KI20A, kinesin-like protein KIF20A; MYO1D, unconventional myosin ID; SMRC1, SWI/SNF complex subunit SMARCC1; UN45A, protein unc-45 homolog A; USO1, general vesicular transport factor p115.
Following co-immunoprecipitation assays of acute myeloid leukemia cells and visualization by electrophoresis, the target band (SPAG6-interacting proteins, between 90 kDa and 130 kDa) was excised and assessed by LC–MS/MS.