Antibodies used in flow cytometry and FlowRNA
| Antibody . | Fluorophore . | Clone . | Company . |
|---|---|---|---|
| Cell surface | |||
| CD3 | AF700 | OKT3 | eBioscience |
| CD3 | APC | SK7 | Becton Dickinson |
| CD4 | ECD | T4 | Beckman Coulter |
| CD8 | AF488 | HIT8a | Biolegend |
| CD8 | BV650 | SK1 | Biolegend |
| CD11b | FITC | ICRF44 | Biolegend |
| CD14 | Pacific blue | M5E2 | Biolegend |
| CD15 | PE/Dazzle 594 | SSEA-1 | Biolegend |
| CD16 | BV650 | 3G8 | Biolegend |
| CD20 | AF700 | 2h7 | Biolegend |
| CD25 | PE | M-A251 | Biolegend |
| CD33 | PE | P67.6 | Biolegend |
| CD38 | PE-Cy7 | HB-7 | Biolegend |
| CD45RA | BV711 | HI100 | Biolegend |
| CD56 | PE | MEM0188 | Thermo Fisher |
| CD56 | ECD | N901 | Beckman Coulter |
| CD197 (CCR7) | PE | G043H7 | Biolegend |
| HLA-DR/DP/DQ | PE-Cy7 | Tu39 | Biolegend |
| TCRγ/δ | PE | B1 | Biolegend |
| Intracellular | |||
| FoxP3 | PE-Cy7 | PCH101 | eBioscience |
| Eomes | PE | WD1928 | eBioscience |
| Perforin | PE-Cy7 | dG9 | Becton Dickinson |
| Ki67 | BV786 | B56 | Becton Dickinson |
| Granzyme B | AF700 | GB11 | Becton Dickinson |
| Antibody . | Fluorophore . | Clone . | Company . |
|---|---|---|---|
| Cell surface | |||
| CD3 | AF700 | OKT3 | eBioscience |
| CD3 | APC | SK7 | Becton Dickinson |
| CD4 | ECD | T4 | Beckman Coulter |
| CD8 | AF488 | HIT8a | Biolegend |
| CD8 | BV650 | SK1 | Biolegend |
| CD11b | FITC | ICRF44 | Biolegend |
| CD14 | Pacific blue | M5E2 | Biolegend |
| CD15 | PE/Dazzle 594 | SSEA-1 | Biolegend |
| CD16 | BV650 | 3G8 | Biolegend |
| CD20 | AF700 | 2h7 | Biolegend |
| CD25 | PE | M-A251 | Biolegend |
| CD33 | PE | P67.6 | Biolegend |
| CD38 | PE-Cy7 | HB-7 | Biolegend |
| CD45RA | BV711 | HI100 | Biolegend |
| CD56 | PE | MEM0188 | Thermo Fisher |
| CD56 | ECD | N901 | Beckman Coulter |
| CD197 (CCR7) | PE | G043H7 | Biolegend |
| HLA-DR/DP/DQ | PE-Cy7 | Tu39 | Biolegend |
| TCRγ/δ | PE | B1 | Biolegend |
| Intracellular | |||
| FoxP3 | PE-Cy7 | PCH101 | eBioscience |
| Eomes | PE | WD1928 | eBioscience |
| Perforin | PE-Cy7 | dG9 | Becton Dickinson |
| Ki67 | BV786 | B56 | Becton Dickinson |
| Granzyme B | AF700 | GB11 | Becton Dickinson |