Table 5.

Expression of cell-surface and cytoplasmic markers for the diagnosis of AML and MPAL

Diagnosis of AML
Diagnosis of AML  
 Precursor marker CD34, CD117, HLA-DR 
 Myeloid markers Cytoplasmic MPO, CD33, CD13 
 Myeloid maturation markers CD11b, CD15, CD64, CD65 
 Monocytic markers CD14, CD36, CD64, CD4, CD38, CD11c 
 Megakaryocytic markers CD41 (glycoprotein IIb/IIIa), CD61 (glycoprotein IIIa), CD36 
 Erythroid markers CD235a (glycophorin A), CD71, CD36 
Diagnosis of MPAL  
 Myeloid lineage MPO (flow cytometry, immunohistochemistry or cytochemistry), or
monocytic differentiation (at least 2 of the following: non-specific esterase cytochemistry, CD11c, CD14, CD64, lysozyme), or
at least two myeloid markers, ie, CD177, CD33, CD13 
 T-lineage Strong cytoplasmic CD3 (with antibodies to CD3 ε chain) or surface CD3 
 B-lineageg Strong CD19 with at least one of the following strongly expressed: cytoplasmic CD79a, cCD22 or CD10, or
weak CD19 with at least two of the following strongly expressed: CD79a, cCD22 or CD10 
Core MRD markers  
 CD34, CD117, CD45, CD33, CD13, CD56, CD7, HLA-DR
If monocytic: CD64, CD11b, CD4 (in addition) 
Diagnosis of AML
Diagnosis of AML  
 Precursor marker CD34, CD117, HLA-DR 
 Myeloid markers Cytoplasmic MPO, CD33, CD13 
 Myeloid maturation markers CD11b, CD15, CD64, CD65 
 Monocytic markers CD14, CD36, CD64, CD4, CD38, CD11c 
 Megakaryocytic markers CD41 (glycoprotein IIb/IIIa), CD61 (glycoprotein IIIa), CD36 
 Erythroid markers CD235a (glycophorin A), CD71, CD36 
Diagnosis of MPAL  
 Myeloid lineage MPO (flow cytometry, immunohistochemistry or cytochemistry), or
monocytic differentiation (at least 2 of the following: non-specific esterase cytochemistry, CD11c, CD14, CD64, lysozyme), or
at least two myeloid markers, ie, CD177, CD33, CD13 
 T-lineage Strong cytoplasmic CD3 (with antibodies to CD3 ε chain) or surface CD3 
 B-lineageg Strong CD19 with at least one of the following strongly expressed: cytoplasmic CD79a, cCD22 or CD10, or
weak CD19 with at least two of the following strongly expressed: CD79a, cCD22 or CD10 
Core MRD markers  
 CD34, CD117, CD45, CD33, CD13, CD56, CD7, HLA-DR
If monocytic: CD64, CD11b, CD4 (in addition) 
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