Expression of cell-surface and cytoplasmic markers for the diagnosis of AML and MPAL
Diagnosis of AML . | . |
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Diagnosis of AML | |
Precursor marker | CD34, CD117, HLA-DR |
Myeloid markers | Cytoplasmic MPO, CD33, CD13 |
Myeloid maturation markers | CD11b, CD15, CD64, CD65 |
Monocytic markers | CD14, CD36, CD64, CD4, CD38, CD11c |
Megakaryocytic markers | CD41 (glycoprotein IIb/IIIa), CD61 (glycoprotein IIIa), CD36 |
Erythroid markers | CD235a (glycophorin A), CD71, CD36 |
Diagnosis of MPAL | |
Myeloid lineage | MPO (flow cytometry, immunohistochemistry or cytochemistry), or monocytic differentiation (at least 2 of the following: non-specific esterase cytochemistry, CD11c, CD14, CD64, lysozyme), or at least two myeloid markers, ie, CD177, CD33, CD13 |
T-lineage | Strong cytoplasmic CD3 (with antibodies to CD3 ε chain) or surface CD3 |
B-lineageg | Strong CD19 with at least one of the following strongly expressed: cytoplasmic CD79a, cCD22 or CD10, or weak CD19 with at least two of the following strongly expressed: CD79a, cCD22 or CD10 |
Core MRD markers | |
CD34, CD117, CD45, CD33, CD13, CD56, CD7, HLA-DR If monocytic: CD64, CD11b, CD4 (in addition) |
Diagnosis of AML . | . |
---|---|
Diagnosis of AML | |
Precursor marker | CD34, CD117, HLA-DR |
Myeloid markers | Cytoplasmic MPO, CD33, CD13 |
Myeloid maturation markers | CD11b, CD15, CD64, CD65 |
Monocytic markers | CD14, CD36, CD64, CD4, CD38, CD11c |
Megakaryocytic markers | CD41 (glycoprotein IIb/IIIa), CD61 (glycoprotein IIIa), CD36 |
Erythroid markers | CD235a (glycophorin A), CD71, CD36 |
Diagnosis of MPAL | |
Myeloid lineage | MPO (flow cytometry, immunohistochemistry or cytochemistry), or monocytic differentiation (at least 2 of the following: non-specific esterase cytochemistry, CD11c, CD14, CD64, lysozyme), or at least two myeloid markers, ie, CD177, CD33, CD13 |
T-lineage | Strong cytoplasmic CD3 (with antibodies to CD3 ε chain) or surface CD3 |
B-lineageg | Strong CD19 with at least one of the following strongly expressed: cytoplasmic CD79a, cCD22 or CD10, or weak CD19 with at least two of the following strongly expressed: CD79a, cCD22 or CD10 |
Core MRD markers | |
CD34, CD117, CD45, CD33, CD13, CD56, CD7, HLA-DR If monocytic: CD64, CD11b, CD4 (in addition) |