Clonality assessment methods
| Assessment method . |
|---|
| For CTCL, T-cell clonality can be established by one of the following molecular methods |
| BIOMED-2 or other PCR-based, approved protocol for both TCR-γ and TCR-β gene rearrangement |
| Next generation sequencing (high-throughput sequencing) |
| For CBCL, B-cell clonality can be established by one of the following molecular methods |
| BIOMED-2 protocol or other PCR-approved method for immunoglobulin heavy chain (IgH) and/or Igκ rearrangement |
| Surface or cytoplasmic light chains restriction by flow cytometry (peripheral blood or tissue samples) |
| Monotypic surface light chains restriction by immunohistochemistry (κ and λ immunostain) |
| Light chains restriction by conventional or RNA-scope ultrasensitive in situ hybridization |
| IgH rearrangement by next generation sequencing for IgH or Igκ |
| Assessment method . |
|---|
| For CTCL, T-cell clonality can be established by one of the following molecular methods |
| BIOMED-2 or other PCR-based, approved protocol for both TCR-γ and TCR-β gene rearrangement |
| Next generation sequencing (high-throughput sequencing) |
| For CBCL, B-cell clonality can be established by one of the following molecular methods |
| BIOMED-2 protocol or other PCR-approved method for immunoglobulin heavy chain (IgH) and/or Igκ rearrangement |
| Surface or cytoplasmic light chains restriction by flow cytometry (peripheral blood or tissue samples) |
| Monotypic surface light chains restriction by immunohistochemistry (κ and λ immunostain) |
| Light chains restriction by conventional or RNA-scope ultrasensitive in situ hybridization |
| IgH rearrangement by next generation sequencing for IgH or Igκ |