Mutational load in WBC subfractions for a selection of community-dwelling subjects with monocytosis and clonal hematopoiesis
| Case . | . | 1 . | 2 . | 3 . | 4 . | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Mutation . | DNMT3A 2245C>T . | SF3B1 1866G>T . | ASXL1 1772dup . | DNMT3A 1711_1720del . | DNMT3A 1811G>T . | DNMT3A 2371del . | SRSF2 284C>A . | TET2 3732_3733del . | ||||||||||||||||
| Fraction . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | |
| 1 | Bulk | 1.6 | 56 | 3500 | 7.4 | 285 | 3851 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
| Granulocytes | 0.9 | 6 | 632 | 4.6 | 139 | 3019 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | |
| T cells | 0.4 | 2 | 538 | 0.0 | 0 | 3736 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | |
| Monocytes | 0.9 | 14 | 1572 | 5.3 | 246 | 4656 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | |
| 2 | Bulk | — | — | — | — | — | — | 1.7 | 22 | 1294 | 1.50 | 60 | 4000 | 1.0 | 62 | 6200 | — | — | — | — | — | — | — | — | — |
| Granulocytes | — | — | — | — | — | — | 0.0 | 0 | 541 | 0 | 0 | 218 | 0.0 | 0 | 280 | — | — | — | — | — | — | — | — | — | |
| T cells | — | — | — | — | — | — | 0.0 | 0 | 1206 | 0 | 0 | 902 | 0.1 | 2 | 1436 | — | — | — | — | — | — | — | — | — | |
| Monocytes | — | — | — | — | — | — | 2.9 | 48 | 1670 | 0 | 0 | 959 | 2.7 | 38 | 1426 | — | — | — | — | — | — | — | — | — | |
| 3 | Bulk | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 1.8 | 30 | 1666 | — | — | — | — | — | — |
| Granulocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 0.0 | 0 | 40 | — | — | — | — | — | — | |
| T cells | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 1.1 | 2 | 182 | — | — | — | — | — | — | |
| Monocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 2.1 | 8 | 380 | — | — | — | — | — | — | |
| 4 | Bulk | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 39.0 | 1742 | 4466 | 34.0 | 2349 | 6 908 |
| Granulocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 60.0 | 266 | 444 | 37.0 | 1870 | 5 056 | |
| T cells | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 0.2 | 2 | 832 | 0.0 | 10 | 12 254 | |
| Monocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 53.0 | 584 | 1104 | 48.0 | 5397 | 11 345 | |
| Case . | . | 1 . | 2 . | 3 . | 4 . | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Mutation . | DNMT3A 2245C>T . | SF3B1 1866G>T . | ASXL1 1772dup . | DNMT3A 1711_1720del . | DNMT3A 1811G>T . | DNMT3A 2371del . | SRSF2 284C>A . | TET2 3732_3733del . | ||||||||||||||||
| Fraction . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | VAF (%) . | Mutant reads . | Total reads . | |
| 1 | Bulk | 1.6 | 56 | 3500 | 7.4 | 285 | 3851 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
| Granulocytes | 0.9 | 6 | 632 | 4.6 | 139 | 3019 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | |
| T cells | 0.4 | 2 | 538 | 0.0 | 0 | 3736 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | |
| Monocytes | 0.9 | 14 | 1572 | 5.3 | 246 | 4656 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | |
| 2 | Bulk | — | — | — | — | — | — | 1.7 | 22 | 1294 | 1.50 | 60 | 4000 | 1.0 | 62 | 6200 | — | — | — | — | — | — | — | — | — |
| Granulocytes | — | — | — | — | — | — | 0.0 | 0 | 541 | 0 | 0 | 218 | 0.0 | 0 | 280 | — | — | — | — | — | — | — | — | — | |
| T cells | — | — | — | — | — | — | 0.0 | 0 | 1206 | 0 | 0 | 902 | 0.1 | 2 | 1436 | — | — | — | — | — | — | — | — | — | |
| Monocytes | — | — | — | — | — | — | 2.9 | 48 | 1670 | 0 | 0 | 959 | 2.7 | 38 | 1426 | — | — | — | — | — | — | — | — | — | |
| 3 | Bulk | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 1.8 | 30 | 1666 | — | — | — | — | — | — |
| Granulocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 0.0 | 0 | 40 | — | — | — | — | — | — | |
| T cells | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 1.1 | 2 | 182 | — | — | — | — | — | — | |
| Monocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 2.1 | 8 | 380 | — | — | — | — | — | — | |
| 4 | Bulk | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 39.0 | 1742 | 4466 | 34.0 | 2349 | 6 908 |
| Granulocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 60.0 | 266 | 444 | 37.0 | 1870 | 5 056 | |
| T cells | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 0.2 | 2 | 832 | 0.0 | 10 | 12 254 | |
| Monocytes | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 53.0 | 584 | 1104 | 48.0 | 5397 | 11 345 | |