Talin-1 regulation mechanisms
| Domains . | Subdomains/sites . | Regulation mechanism . | Functional outcomes . |
|---|---|---|---|
| Talin head | F3 | Binds to proximal NPxY in β cytoplasmic tail | Topology change of β, separation of α and β subunits, leading to activation |
| Masked by talin-R9 | Autoinhibition | ||
| Binds RIAM (Rap1-RIAM-talin pathway) | Activation | ||
| PIP2 binding sites in F2F3 | Masked by R12 at rest. Bind PIP2 | Autoinhibition; binds PIP2 to activate integrins and anchor to the membrane | |
| Actin binding site 1 (ABS1) in F2F3 | Binds actin | Anchors to cytoskeleton, mechanotransduction | |
| F0F1 | Direct Rap1 binding | Talin recruitment to membrane | |
| F1 flexible loop | (1) Binds to lipid via R146, R153, K156; (2) interferes with a salt bridge in the IMC | Talin recruitment to membrane | |
| Talin rod | Vinculin binding site in R1, 2, 3, 6, 7, 8, 10, 11, 13 | Binds vinculin | Forming vinculin-talin-integrin complex to induce FA formation |
| ABS2, ABS3 in R4-R8 | Binds actin | Anchors to cytoskeleton, mechanotransduction | |
| RIAM binding region in R2, 3, 8, and 11 | RIAM binding | Facilitates talin recruitment to membrane, important for cell migration | |
| R7 | Kank-1/2 binding | Activates talin | |
| R8 | Paxillin binding; DLC-1 binding | FA assembly and cell migration |
| Domains . | Subdomains/sites . | Regulation mechanism . | Functional outcomes . |
|---|---|---|---|
| Talin head | F3 | Binds to proximal NPxY in β cytoplasmic tail | Topology change of β, separation of α and β subunits, leading to activation |
| Masked by talin-R9 | Autoinhibition | ||
| Binds RIAM (Rap1-RIAM-talin pathway) | Activation | ||
| PIP2 binding sites in F2F3 | Masked by R12 at rest. Bind PIP2 | Autoinhibition; binds PIP2 to activate integrins and anchor to the membrane | |
| Actin binding site 1 (ABS1) in F2F3 | Binds actin | Anchors to cytoskeleton, mechanotransduction | |
| F0F1 | Direct Rap1 binding | Talin recruitment to membrane | |
| F1 flexible loop | (1) Binds to lipid via R146, R153, K156; (2) interferes with a salt bridge in the IMC | Talin recruitment to membrane | |
| Talin rod | Vinculin binding site in R1, 2, 3, 6, 7, 8, 10, 11, 13 | Binds vinculin | Forming vinculin-talin-integrin complex to induce FA formation |
| ABS2, ABS3 in R4-R8 | Binds actin | Anchors to cytoskeleton, mechanotransduction | |
| RIAM binding region in R2, 3, 8, and 11 | RIAM binding | Facilitates talin recruitment to membrane, important for cell migration | |
| R7 | Kank-1/2 binding | Activates talin | |
| R8 | Paxillin binding; DLC-1 binding | FA assembly and cell migration |
FA, focal adhesion.