Structural parameters for the cryo-EM structures of fV and fVa
| . | Prothrombin-fVa-fXa complex (PDB ID:7TPP emd ID:26060) . | fXa-fVa complex on nanodiscs (PDB ID:7TPQ emd ID:26061) . |
|---|---|---|
| Map | ||
| Voltage (kV) | 300 | 200 |
| Electron exposure (e-/Å2) | 50 | 51.28 |
| Pixel size (Å) | 0.413 | 0.94 |
| Initial particle number | 12260048 | 572677 |
| Final particle number | 330317 | 16853 |
| Symmetry imposed | C1 | C1 |
| Resolution (unmasked, FSC threshold 0.143) | 5.2 | 12.1 |
| Resolution (masked, FSC threshold 0.143) | 4.1 | 5.3 |
| Model | ||
| Refinement program | Phenix (real space) | Phenix (real space) |
| Number of protein atoms (non-H) | 18513 | 14020 |
| RMSD bonds (Å) | 0.003 | 0.003 |
| RMSD angles (°) | 0.894 | 0.921 |
| Ramachandran favored (%) | 75.36 | 76.37 |
| Ramachandran allowed (%) | 21.81 | 20.52 |
| Ramachandran disallowed (%) | 2.83 | 3.11 |
| All-atom clash score | 9.9 | 12.3 |
| MolProbity score | 2.66 | 2.78 |
| . | Prothrombin-fVa-fXa complex (PDB ID:7TPP emd ID:26060) . | fXa-fVa complex on nanodiscs (PDB ID:7TPQ emd ID:26061) . |
|---|---|---|
| Map | ||
| Voltage (kV) | 300 | 200 |
| Electron exposure (e-/Å2) | 50 | 51.28 |
| Pixel size (Å) | 0.413 | 0.94 |
| Initial particle number | 12260048 | 572677 |
| Final particle number | 330317 | 16853 |
| Symmetry imposed | C1 | C1 |
| Resolution (unmasked, FSC threshold 0.143) | 5.2 | 12.1 |
| Resolution (masked, FSC threshold 0.143) | 4.1 | 5.3 |
| Model | ||
| Refinement program | Phenix (real space) | Phenix (real space) |
| Number of protein atoms (non-H) | 18513 | 14020 |
| RMSD bonds (Å) | 0.003 | 0.003 |
| RMSD angles (°) | 0.894 | 0.921 |
| Ramachandran favored (%) | 75.36 | 76.37 |
| Ramachandran allowed (%) | 21.81 | 20.52 |
| Ramachandran disallowed (%) | 2.83 | 3.11 |
| All-atom clash score | 9.9 | 12.3 |
| MolProbity score | 2.66 | 2.78 |