Table 2. K D,app binding constants... | American Society of Hematology

Table 2.

K_D,app binding constants measured for WT and mutant GPVI-Fc binding to collagenous substrates in solid-phase plate-binding assays

Mutant	CRP			Fibrillar collagen I			Toolkit peptide III-30
Mutant	K_D,app (nM)	95% CI (nM)	Fold increase	K_D,app (nM)	95% CI (nM)	Fold increase	K_D,app (nM)	95% CI (nM)	Fold increase
WT	2.71	2.2-3.2		30.1	26-34		10.9	9.7-12.0
E40A	2.5 × 10³	2.1-2.8 × 10³	913	25 × 10³	22.1-27.5 × 10³	823	74 × 10³	57-91 × 10³	6848
R38A	2.0 × 10³	1.7-2.3 × 10³	748	18 × 10³	16.0-20.3 × 10³	603	42 × 10³	38-45 × 10³	3833
W76A	2.0 × 10³	1.8-2.2 × 10³	744	43 × 10³	37.2-49.6 × 10³	1440	21 × 10³	14-27 × 10³	1905
D49A	599	454-744	221	9.2 × 10³	8.03-10.4 × 10³	306	3.9 × 10³	3.55-4.19 × 10³	357
R67A	238	187-289	88	10 × 10³	6.63-12.9 × 10³	324	2.0 × 10³	1.90-2.16 × 10³	187
Y47A	160	130-189	59	6.0 × 10³	4.43-7.56 × 10³	199	10 × 10³	8.5-12 × 10³	925
S69A	45.7	24-67	17	1.2 × 10³	0.93-1.49 × 10³	40	6.5 × 10³	5.66-7.40 × 10³	602
Q71A	19.9	11-29	7	72.2	54.8-89.5	2	9.15*	6.87-11.4	0.84
L36A	18.8	12-25	7	186	150-222	6	7.68*	5.77-9.58	0.70
Q82A	3.89	2.5-5.3	1.4	433	329-538	14	289	268-310	27

Mutant	CRP			Fibrillar collagen I			Toolkit peptide III-30
Mutant	K_D,app (nM)	95% CI (nM)	Fold increase	K_D,app (nM)	95% CI (nM)	Fold increase	K_D,app (nM)	95% CI (nM)	Fold increase
WT	2.71	2.2-3.2		30.1	26-34		10.9	9.7-12.0
E40A	2.5 × 10³	2.1-2.8 × 10³	913	25 × 10³	22.1-27.5 × 10³	823	74 × 10³	57-91 × 10³	6848
R38A	2.0 × 10³	1.7-2.3 × 10³	748	18 × 10³	16.0-20.3 × 10³	603	42 × 10³	38-45 × 10³	3833
W76A	2.0 × 10³	1.8-2.2 × 10³	744	43 × 10³	37.2-49.6 × 10³	1440	21 × 10³	14-27 × 10³	1905
D49A	599	454-744	221	9.2 × 10³	8.03-10.4 × 10³	306	3.9 × 10³	3.55-4.19 × 10³	357
R67A	238	187-289	88	10 × 10³	6.63-12.9 × 10³	324	2.0 × 10³	1.90-2.16 × 10³	187
Y47A	160	130-189	59	6.0 × 10³	4.43-7.56 × 10³	199	10 × 10³	8.5-12 × 10³	925
S69A	45.7	24-67	17	1.2 × 10³	0.93-1.49 × 10³	40	6.5 × 10³	5.66-7.40 × 10³	602
Q71A	19.9	11-29	7	72.2	54.8-89.5	2	9.15*	6.87-11.4	0.84
L36A	18.8	12-25	7	186	150-222	6	7.68*	5.77-9.58	0.70
Q82A	3.89	2.5-5.3	1.4	433	329-538	14	289	268-310	27

Maximum III-30-binding of the Q71A and L36A mutants is 109% (104-114%) compared with WT protein.