Primers used for qPCR to determine mitochondrial copy #
| Mitochondrial . | Primer . | Sequence . |
|---|---|---|
| ND1 (JS) | Forward | CTAGCAGAAACAAACCGGGC |
| Reverse | CCGGCTGCGTATTCTACGTT | |
| ND3 (TB) | Forward | GCATTCTGACTCCCCCAAAT |
| Reverse | TGAATTGCTCATGGTAGTGGA | |
| 12S (TB) | Forward | ACCGCGGTCATACGATTAAC |
| Reverse | CCCAGTTTGGGTCTTAGCTG | |
| 16S (JS) | Forward | GCAATTATTCCCCATGAACG |
| Reverse | CCGCAAGGGAAAGATGAAAGAC |
| Mitochondrial . | Primer . | Sequence . |
|---|---|---|
| ND1 (JS) | Forward | CTAGCAGAAACAAACCGGGC |
| Reverse | CCGGCTGCGTATTCTACGTT | |
| ND3 (TB) | Forward | GCATTCTGACTCCCCCAAAT |
| Reverse | TGAATTGCTCATGGTAGTGGA | |
| 12S (TB) | Forward | ACCGCGGTCATACGATTAAC |
| Reverse | CCCAGTTTGGGTCTTAGCTG | |
| 16S (JS) | Forward | GCAATTATTCCCCATGAACG |
| Reverse | CCGCAAGGGAAAGATGAAAGAC |
| Genomic . | Primer . | Sequence . |
|---|---|---|
| 18S (TB) | Forward | CGCGGTTCTATTTTGTTGGT |
| Reverse | AGTCGGCATCGTTTATGGTC | |
| SEMA (GS) | Forward | ACCGATTCCAGATGATTGGC |
| Reverse | TCCATATTAATGCAGTGCTTGC | |
| HK2 (JS) | Forward | GCCAGCCTCTCCTGATTTTAGTGT |
| Reverse | GGGAACACAAAAGACCTCTTCTGG |
| Genomic . | Primer . | Sequence . |
|---|---|---|
| 18S (TB) | Forward | CGCGGTTCTATTTTGTTGGT |
| Reverse | AGTCGGCATCGTTTATGGTC | |
| SEMA (GS) | Forward | ACCGATTCCAGATGATTGGC |
| Reverse | TCCATATTAATGCAGTGCTTGC | |
| HK2 (JS) | Forward | GCCAGCCTCTCCTGATTTTAGTGT |
| Reverse | GGGAACACAAAAGACCTCTTCTGG |