Ptpn11T468M/+ mouse characteristics
| . | Mean ± SEM . | |
|---|---|---|
| WT . | Ptpn11T468M/+ . | |
| Platelet count, ×109/L* | 707 ± 22 | 638 ± 24† |
| MPV, µm3* | 7.4 ± 0.3 | 6.7 ± 0.3 |
| Fibrinogen, g/L‡ | 1.1 ± 0.1 | 1.1 ± 0.1 |
| PR, %‡ | 140 ± 10 | 142 ± 7 |
| aPTT, s‡ | 34.3 ± 2.4 | 39.0 ± 1.0 |
| GPIb, MFI§ | 7242 ± 273 | 8422 ± 1057 |
| GPIIb, MFI§ | 9335 ± 427 | 9230 ± 212 |
| CLEC-2, MFI§ | 4693 ± 528 | 5782 ± 1085 |
| α2, MFI§ | 51 ± 6 | 49 ± 4 |
| GPVI, MFI§ | 1743 ± 93 | 1552 ± 78 |
| . | Mean ± SEM . | |
|---|---|---|
| WT . | Ptpn11T468M/+ . | |
| Platelet count, ×109/L* | 707 ± 22 | 638 ± 24† |
| MPV, µm3* | 7.4 ± 0.3 | 6.7 ± 0.3 |
| Fibrinogen, g/L‡ | 1.1 ± 0.1 | 1.1 ± 0.1 |
| PR, %‡ | 140 ± 10 | 142 ± 7 |
| aPTT, s‡ | 34.3 ± 2.4 | 39.0 ± 1.0 |
| GPIb, MFI§ | 7242 ± 273 | 8422 ± 1057 |
| GPIIb, MFI§ | 9335 ± 427 | 9230 ± 212 |
| CLEC-2, MFI§ | 4693 ± 528 | 5782 ± 1085 |
| α2, MFI§ | 51 ± 6 | 49 ± 4 |
| GPVI, MFI§ | 1743 ± 93 | 1552 ± 78 |
MFI, mean fluorescence intensity; MPV, mean platelet volume.
Whole-blood platelet count and MPV were measured by using a HORIBA ABX Micros 60 analyzer (n = 22 mice of each genotype).
P < .001 vs WT according to Student t test.
PR, aPTT, ratio, and fibrinogen concentration were measured as described in “Methods” (n = 5 mice of each genotype).
Platelet surface expressions of GPIb, GPIIb, CLEC-2, αII, and GPVI were measured by flow cytometry using specific antibodies (n = 6 mice of each genotype).