Ptpn11D61G/+ mouse characteristics
| . | Mean ± SEM . | |
|---|---|---|
| WT . | Ptpn11D61G/+ . | |
| Platelet count, ×109/L* | 647 ± 22 | 513 ± 29† |
| MPV, µm3* | 6.8 ± 0.3 | 6.2 ± 0.3 |
| Fibrinogen, g/L‡ | 1.9 ± 0.1 | 2.1 ± 0.1 |
| PR, %‡ | 133 ± 5 | 170 ± 10 |
| aPTT, s‡ | 27.8 ± 0.5 | 31.9 ± 2.7 |
| GPIb, MFI§ | 2569 ± 222 | 2954 ± 277 |
| GPIIb, MFI§ | 8749 ± 261 | 8312 ± 394 |
| CLEC-2, MFI§ | 5365 ± 258 | 6018 ± 463 |
| α2, MFI§ | 55 ± 7 | 44 ± 2 |
| GPVI, MFI§ | 1336 ± 62 | 1465 ± 78 |
| . | Mean ± SEM . | |
|---|---|---|
| WT . | Ptpn11D61G/+ . | |
| Platelet count, ×109/L* | 647 ± 22 | 513 ± 29† |
| MPV, µm3* | 6.8 ± 0.3 | 6.2 ± 0.3 |
| Fibrinogen, g/L‡ | 1.9 ± 0.1 | 2.1 ± 0.1 |
| PR, %‡ | 133 ± 5 | 170 ± 10 |
| aPTT, s‡ | 27.8 ± 0.5 | 31.9 ± 2.7 |
| GPIb, MFI§ | 2569 ± 222 | 2954 ± 277 |
| GPIIb, MFI§ | 8749 ± 261 | 8312 ± 394 |
| CLEC-2, MFI§ | 5365 ± 258 | 6018 ± 463 |
| α2, MFI§ | 55 ± 7 | 44 ± 2 |
| GPVI, MFI§ | 1336 ± 62 | 1465 ± 78 |
aPTT, activated partial thromboplastin time; MFI, mean fluorescence intensity; MPV, mean platelet volume; PR, prothrombin ratio.
Whole-blood platelet count and MPV were measured by using a HORIBA ABX Micros 60 analyzer (n = 22 mice of each genotype).
P < .001 vs WT according to Student t test.
PR, aPTT ratio, and fibrinogen concentration were measured as described in “Methods” (n = 5 mice of each genotype).
Platelet surface expressions of GPIb, GPIIb, CLEC-2, α2, and GPVI were measured by flow cytometry using specific antibodies (n = 6 mice of each genotype).