Evaluation of binding of WT protein S and composite protein S variants to C4BP
| Protein S variant . | Kd (nM) . |
|---|---|
| WT | 0.67 ± 0.18 |
| LG1A | 1.21 ± 0.31** |
| LG1B | 1.13 ± 0.37* |
| LG1C | 1.40 ± 0.35** |
| Protein S variant . | Kd (nM) . |
|---|---|
| WT | 0.67 ± 0.18 |
| LG1A | 1.21 ± 0.31** |
| LG1B | 1.13 ± 0.37* |
| LG1C | 1.40 ± 0.35** |
Serial dilutions (0-20 nM) of protein S were incubated with 5 µg/mL plate-bound C4BP. Bound protein S was detected with monoclonal antiprotein S antibodies. The affinity of the interaction was derived using a 1-site binding equation. The average of Kd values is shown as mean ± SD (n = 6).
P < .05, **P < .01; evaluated by Mann Whitney test.