Product characterization
Patient ID . | Percentage . | SFC/1 × 105 T cells . | Fold expansion . | Total no. of cells frozen . | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
B cells . | T cells . | CD4+ . | CD8+ . | Natural Killer T-cells . | Natural killer cells . | CD3+ TCRab . | CD3+ TCRgd . | Mono . | DCs . | Actin . | WT1 . | Prame . | Survivin . | TAA* . | |||
Patient 1 | 0 | 99.5 | 10.9 | 29.3 | 42 | 0.92 | 16.3 | 73.5 | 0.3 | 0 | 7 | 16 | 179.5 | 2 | 88 | 23.2 | 1.39e8 |
Patient 2 | 1.58 | 84.9 | 10.2 | 74.6 | 52.3 | 0.52 | 80.3 | 4.1 | 1.08 | 0.16 | 1 | 2.5 | 5 | 0 | 5.5 | 12.3 | 2.45107 |
Patient 3 | 0 | 84.5 | 20 | 39 | 25 | 0.3 | 66 | 23.1 | 0 | 0 | 154.5 | 139 | 335 | 132.5 | 445 | 5 | 1.36e8 |
Patient 4 | 0.2 | 99.1 | 16.9 | 81.6 | 2.2 | 14.8 | 91.8 | 69 | 0.2 | 0 | 5.5 | 10.5 | 72.5 | 5 | 48 | 5.91 | 1e8 |
Patient 5 | 0.05 | 83.3 | 1.74 | 42.7 | 23.7 | 16.8 | 45.3 | 42.2 | 0.01 | 0.04 | 3.5 | 5 | 4 | 1 | 25.5 | 4.92 | 9.35107 |
Patient 6 | 0.1 | 80.9 | 4.8 | 69.8 | 27.9 | 4.1 | 82.7 | 12.3 | 0 | 0 | 2.5 | 16 | 234 | 2 | 182.5 | 3.86 | 1.39108 |
Patient 6_2 | 0 | 97.3 | 7.1 | 77 | 58.3 | 2.6 | 71.6 | 26 | 0 | 0 | 7.5 | 15 | 12 | 5 | 27 | 4.1 | 4.95 × 107 |
Patient 7 | 0.07 | 96 | 20 | 75.7 | 45.1 | 1.1 | 87.4 | 11.5 | 0 | 0.02 | 18.5 | 24.5 | 15.5 | 30 | 20.5 | 5.57 | 1.3e8 |
Patient 7_2 | 0 | 99.3 | 10.4 | 85.4 | 1.44 | 0.17 | 94..1 | 4 | 0 | 0 | 5.5 | 1 | 3 | 6 | 9 | 1.83 | 1.7 × 107 |
Patient 8 | 0.3 | 98 | 1.73 | 43.3 | 33.7 | 0.6 | 39.5 | 58 | 0.1 | 0 | 7.5 | 37 | 67 | 18 | 22 | 5.97 | 1.88e8 |
Patient 9 | 0.05 | 98.3 | 9.5 | 87.5 | 11.2 | 0 | 97.5 | 1.9 | 0 | 0.02 | 9.5 | 11 | 16 | 9 | 11.5 | 6.52 | 1.37e8 |
Patient 10 | 0.09 | 99.5 | 11.8 | 69.5 | 3.59 | 0 | 81.4 | 16.1 | 0 | 0 | 1.5 | 15.5 | 1 | 14 | 10.5 | 2.31 | 4.38107 |
Patient ID . | Percentage . | SFC/1 × 105 T cells . | Fold expansion . | Total no. of cells frozen . | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
B cells . | T cells . | CD4+ . | CD8+ . | Natural Killer T-cells . | Natural killer cells . | CD3+ TCRab . | CD3+ TCRgd . | Mono . | DCs . | Actin . | WT1 . | Prame . | Survivin . | TAA* . | |||
Patient 1 | 0 | 99.5 | 10.9 | 29.3 | 42 | 0.92 | 16.3 | 73.5 | 0.3 | 0 | 7 | 16 | 179.5 | 2 | 88 | 23.2 | 1.39e8 |
Patient 2 | 1.58 | 84.9 | 10.2 | 74.6 | 52.3 | 0.52 | 80.3 | 4.1 | 1.08 | 0.16 | 1 | 2.5 | 5 | 0 | 5.5 | 12.3 | 2.45107 |
Patient 3 | 0 | 84.5 | 20 | 39 | 25 | 0.3 | 66 | 23.1 | 0 | 0 | 154.5 | 139 | 335 | 132.5 | 445 | 5 | 1.36e8 |
Patient 4 | 0.2 | 99.1 | 16.9 | 81.6 | 2.2 | 14.8 | 91.8 | 69 | 0.2 | 0 | 5.5 | 10.5 | 72.5 | 5 | 48 | 5.91 | 1e8 |
Patient 5 | 0.05 | 83.3 | 1.74 | 42.7 | 23.7 | 16.8 | 45.3 | 42.2 | 0.01 | 0.04 | 3.5 | 5 | 4 | 1 | 25.5 | 4.92 | 9.35107 |
Patient 6 | 0.1 | 80.9 | 4.8 | 69.8 | 27.9 | 4.1 | 82.7 | 12.3 | 0 | 0 | 2.5 | 16 | 234 | 2 | 182.5 | 3.86 | 1.39108 |
Patient 6_2 | 0 | 97.3 | 7.1 | 77 | 58.3 | 2.6 | 71.6 | 26 | 0 | 0 | 7.5 | 15 | 12 | 5 | 27 | 4.1 | 4.95 × 107 |
Patient 7 | 0.07 | 96 | 20 | 75.7 | 45.1 | 1.1 | 87.4 | 11.5 | 0 | 0.02 | 18.5 | 24.5 | 15.5 | 30 | 20.5 | 5.57 | 1.3e8 |
Patient 7_2 | 0 | 99.3 | 10.4 | 85.4 | 1.44 | 0.17 | 94..1 | 4 | 0 | 0 | 5.5 | 1 | 3 | 6 | 9 | 1.83 | 1.7 × 107 |
Patient 8 | 0.3 | 98 | 1.73 | 43.3 | 33.7 | 0.6 | 39.5 | 58 | 0.1 | 0 | 7.5 | 37 | 67 | 18 | 22 | 5.97 | 1.88e8 |
Patient 9 | 0.05 | 98.3 | 9.5 | 87.5 | 11.2 | 0 | 97.5 | 1.9 | 0 | 0.02 | 9.5 | 11 | 16 | 9 | 11.5 | 6.52 | 1.37e8 |
Patient 10 | 0.09 | 99.5 | 11.8 | 69.5 | 3.59 | 0 | 81.4 | 16.1 | 0 | 0 | 1.5 | 15.5 | 1 | 14 | 10.5 | 2.31 | 4.38107 |
TAA represents spot-forming cells (SFC) per 1 × 105 T cells in response to a mixture of WT1, PRAME, and Survivn added to the same experimental well.