IGHV4-34 gene usage in t(14;18)-positive and (14;18)-negative FL at primary diagnosis with clonal IGHV gene amplification and either newly acquired N-glycosylation sites (NANGS) or IGHV4-34 usage
| ID . | Stage . | Clonal . | SHM (%) . | Clonal frequency (%) . | IGHV usage . | NANGS . | Natural site . | Natural site lost . |
|---|---|---|---|---|---|---|---|---|
| t(14;18)-negative FL | ||||||||
| 18 | I/II | Yes | 7.0 | 74.0 | '4-59 | 1 | No | No |
| 25 | I/II | Yes | 7.9 | 12.0 | '4-34 | 1 | Yes | No |
| 26 | I/II | Yes | 8.6 | 99.0 | '4-34 | 2 | No | Yes |
| 27 | I/II | Yes | 22.8 | 73.0 | '3-23 | 1 | No | No |
| 28 | I/II | Yes | 38.3 | 21.0 | '1-69 | 2 | No | No |
| 29 | I/II | Yes | 14.0 | 92.0 | '4-34 | 1 | No | Yes |
| 30 | I/II | Yes | 27.7 | 17.9 | '1-69 | 2 | No | No |
| 38 | III/IV | Yes | 15.5 | 97.0 | '4-34 | 1 | No | Yes |
| 39 | III/IV | Yes | 3.6 | 95.0 | '4-34 | 1 | Yes | No |
| 37 | III/IV | Yes | 7.7 | 38.0 | '4-34 | 0 | Yes | No |
| t(14;18)-positive FL | ||||||||
| 1 | I/II | Yes | 21.8 | 65.0 | '3-53 | 1 | No | No |
| 2 | I/II | Yes | 19.3 | 69.0 | '3-21 | 2 | No | No |
| 3 | I/II | Yes | 21.5 | 55.0 | '3-74 | 1 | No | No |
| 4 | I/II | Yes | 12.4 | 28.0 | '4-39 | 1 | No | No |
| 7 | I/II | Yes | 12.8 | 97.0 | '3-48 | 2 | No | No |
| 9 | I/II | Yes | 15.0 | 67.0 | '4-59 | 2 | No | No |
| 43 | III/IV | Yes | 17.9 | 22.0 | '1-2 | 1 | No | No |
| 52 | III/IV | Yes | 7.8 | 80.0 | '3-48 | 1 | No | No |
| 58 | III/IV | Yes | 9.1 | 100.0 | '3-30 | 1 | No | No |
| 59 | III/IV | Yes | 7.8 | 98.0 | '3-11 | 1 | No | No |
| 68 | III/IV | Yes | 4.6 | 14.0 | '4-34 | 0 | Yes | No |
| 72 | III/IV | Yes | 12.3 | 91.0 | '4-34 | 0 | No | Yes |
| ID . | Stage . | Clonal . | SHM (%) . | Clonal frequency (%) . | IGHV usage . | NANGS . | Natural site . | Natural site lost . |
|---|---|---|---|---|---|---|---|---|
| t(14;18)-negative FL | ||||||||
| 18 | I/II | Yes | 7.0 | 74.0 | '4-59 | 1 | No | No |
| 25 | I/II | Yes | 7.9 | 12.0 | '4-34 | 1 | Yes | No |
| 26 | I/II | Yes | 8.6 | 99.0 | '4-34 | 2 | No | Yes |
| 27 | I/II | Yes | 22.8 | 73.0 | '3-23 | 1 | No | No |
| 28 | I/II | Yes | 38.3 | 21.0 | '1-69 | 2 | No | No |
| 29 | I/II | Yes | 14.0 | 92.0 | '4-34 | 1 | No | Yes |
| 30 | I/II | Yes | 27.7 | 17.9 | '1-69 | 2 | No | No |
| 38 | III/IV | Yes | 15.5 | 97.0 | '4-34 | 1 | No | Yes |
| 39 | III/IV | Yes | 3.6 | 95.0 | '4-34 | 1 | Yes | No |
| 37 | III/IV | Yes | 7.7 | 38.0 | '4-34 | 0 | Yes | No |
| t(14;18)-positive FL | ||||||||
| 1 | I/II | Yes | 21.8 | 65.0 | '3-53 | 1 | No | No |
| 2 | I/II | Yes | 19.3 | 69.0 | '3-21 | 2 | No | No |
| 3 | I/II | Yes | 21.5 | 55.0 | '3-74 | 1 | No | No |
| 4 | I/II | Yes | 12.4 | 28.0 | '4-39 | 1 | No | No |
| 7 | I/II | Yes | 12.8 | 97.0 | '3-48 | 2 | No | No |
| 9 | I/II | Yes | 15.0 | 67.0 | '4-59 | 2 | No | No |
| 43 | III/IV | Yes | 17.9 | 22.0 | '1-2 | 1 | No | No |
| 52 | III/IV | Yes | 7.8 | 80.0 | '3-48 | 1 | No | No |
| 58 | III/IV | Yes | 9.1 | 100.0 | '3-30 | 1 | No | No |
| 59 | III/IV | Yes | 7.8 | 98.0 | '3-11 | 1 | No | No |
| 68 | III/IV | Yes | 4.6 | 14.0 | '4-34 | 0 | Yes | No |
| 72 | III/IV | Yes | 12.3 | 91.0 | '4-34 | 0 | No | Yes |
Natural site, located in CDR2 of the IGHV4-34 gene, AA motive NHS. SHM, somatic hypermutations