Effect of EPO injection on hematopoietic stem/progenitor cells, erythroid progenitors, and erythroblasts
| Proportion in BM . | No. in BM . | ||||||
|---|---|---|---|---|---|---|---|
| Cell type . | PBS . | EPO . | P . | Cell type . | PBS . | EPO . | P . |
| HSC (/106) | 3.55 ± 0.48 | 6.02 ± 0.73 | .02541 | HSC (×102) | 4.57 ± 0.52 | 9.16 ± 1.40 | .02200 |
| EpoR-tdTomato+ MPP (/106) | 3.66 ± 0.88 | 13.66 ± 3.07 | .02137 | EpoR-tdTomato+ MPP (×103) | 0.47 ± 0.12 | 1.92 ± 0.38 | .01200 |
| EpoR-tdTomato– MPP (/106) | 33.07 ± 6.99 | 95.25 ± 20.41 | .02857 | EpoR-tdTomato– MPP (×103) | 4.28 ± 0.93 | 13.55 ± 2.53 | .01513 |
| EpoR-tdTomato+ CMP (/103) | 0.05 ± 0.01 | 0.12 ± 0.02 | .00984 | EpoR-tdTomato+ CMP (×104) | 0.86 ± 0.03 | 1.934 ± 0.20 | .00132 |
| EpoR-tdTomato– CMP (/103) | 0.22 ± 0.05 | 0.36 ± 0.04 | .03540 | EpoR-tdTomato– CMP (×104) | 3.68 ± 0.29 | 5.92 ± 0.49 | .00191 |
| GMP (/103) | 0.54 ± 0.08 | 0.26 ± 0.03 | .00863 | GMP (×104) | 7.56 ± 0.78 | 3.72 ± 0.41 | .00326 |
| MEP (/103) | 0.51 ± 0.09 | 1.34 ± 0.18 | .00433 | MEP (×104) | 6.88 ± 1.32 | 18.73 ± 2.55 | .00370 |
| CLP (/106) | 4.66 ± 0.37 | 2.06 ± 0.10 | .03809 | CLP (×102) | 6.99 ± 1.14 | 2.88 ± 0.78 | .03564 |
| BFU-E (/104) | 0.24 ± 0.03 | 0.12 ± 0.01 | .03541 | BFU-E (×104) | 2.94 ± 0.38 | 1.93 ± 0.27 | .09798 |
| CFU-E (/104) | 0.19 ± 0.03 | 0.88 ± 0.06 | .00062 | CFU-E (×104) | 2.49 ± 0.38 | 13.7 ± 0.12 | .00090 |
| Erythroblasts (/102) | 17.87 ± 0.93 | 33.28 ± 1.99 | .00039 | Erythroblasts (×106) | 23.15 ± 1.59 | 50.07 ± 2.64 | .00010 |
| Proportion in BM . | No. in BM . | ||||||
|---|---|---|---|---|---|---|---|
| Cell type . | PBS . | EPO . | P . | Cell type . | PBS . | EPO . | P . |
| HSC (/106) | 3.55 ± 0.48 | 6.02 ± 0.73 | .02541 | HSC (×102) | 4.57 ± 0.52 | 9.16 ± 1.40 | .02200 |
| EpoR-tdTomato+ MPP (/106) | 3.66 ± 0.88 | 13.66 ± 3.07 | .02137 | EpoR-tdTomato+ MPP (×103) | 0.47 ± 0.12 | 1.92 ± 0.38 | .01200 |
| EpoR-tdTomato– MPP (/106) | 33.07 ± 6.99 | 95.25 ± 20.41 | .02857 | EpoR-tdTomato– MPP (×103) | 4.28 ± 0.93 | 13.55 ± 2.53 | .01513 |
| EpoR-tdTomato+ CMP (/103) | 0.05 ± 0.01 | 0.12 ± 0.02 | .00984 | EpoR-tdTomato+ CMP (×104) | 0.86 ± 0.03 | 1.934 ± 0.20 | .00132 |
| EpoR-tdTomato– CMP (/103) | 0.22 ± 0.05 | 0.36 ± 0.04 | .03540 | EpoR-tdTomato– CMP (×104) | 3.68 ± 0.29 | 5.92 ± 0.49 | .00191 |
| GMP (/103) | 0.54 ± 0.08 | 0.26 ± 0.03 | .00863 | GMP (×104) | 7.56 ± 0.78 | 3.72 ± 0.41 | .00326 |
| MEP (/103) | 0.51 ± 0.09 | 1.34 ± 0.18 | .00433 | MEP (×104) | 6.88 ± 1.32 | 18.73 ± 2.55 | .00370 |
| CLP (/106) | 4.66 ± 0.37 | 2.06 ± 0.10 | .03809 | CLP (×102) | 6.99 ± 1.14 | 2.88 ± 0.78 | .03564 |
| BFU-E (/104) | 0.24 ± 0.03 | 0.12 ± 0.01 | .03541 | BFU-E (×104) | 2.94 ± 0.38 | 1.93 ± 0.27 | .09798 |
| CFU-E (/104) | 0.19 ± 0.03 | 0.88 ± 0.06 | .00062 | CFU-E (×104) | 2.49 ± 0.38 | 13.7 ± 0.12 | .00090 |
| Erythroblasts (/102) | 17.87 ± 0.93 | 33.28 ± 1.99 | .00039 | Erythroblasts (×106) | 23.15 ± 1.59 | 50.07 ± 2.64 | .00010 |
Multicolor staining of HSCs and various progenitors were performed by using the enriched lineage– cells. The proportion of HSCs and various progenitor cells in BM was calculated by: proportion of the enriched lineage– cells in total BM cells × proportion of the target cell cluster in the lineage– cells. The numbers of each cell population were calculated by: proportion in BM cells × total BM cells. N = 3. PBS, phosphate-buffered saline.