Overview of the purified anti-CS and anti-CUB autoantibodies from 13 iTTP patients
Patient code . | ID . | Antibody concentration (µg/mL) . | |
---|---|---|---|
Anti-CS . | Anti-CUB . | ||
A | MF-KB-TTP01-A | 64 | 108 |
B | MF-KB-TTP02-A | 58 | |
C | MF-KB-TTP03-A | 56 | |
D | MF-KB-TTP05-A | 101 | |
E | MF-KB-TTP09-A | 35 | |
F | MF-KB-TTP10-A | 50 | 73* |
G | MF-KB-TTP11-A | 114 | |
H | MF-KB-TTP12-A | 124 | |
I | MF-KB-TTP13-A | 75 | |
J | MF-KB-TTP15-A | 30 | |
K | MF-KB-TTP17-A | 51 | |
L | MF-KB-TTP18-A | ||
M | MF-KB-TTP20-A | 69 | 214 |
Patient code . | ID . | Antibody concentration (µg/mL) . | |
---|---|---|---|
Anti-CS . | Anti-CUB . | ||
A | MF-KB-TTP01-A | 64 | 108 |
B | MF-KB-TTP02-A | 58 | |
C | MF-KB-TTP03-A | 56 | |
D | MF-KB-TTP05-A | 101 | |
E | MF-KB-TTP09-A | 35 | |
F | MF-KB-TTP10-A | 50 | 73* |
G | MF-KB-TTP11-A | 114 | |
H | MF-KB-TTP12-A | 124 | |
I | MF-KB-TTP13-A | 75 | |
J | MF-KB-TTP15-A | 30 | |
K | MF-KB-TTP17-A | 51 | |
L | MF-KB-TTP18-A | ||
M | MF-KB-TTP20-A | 69 | 214 |
Patient code, patient ID, and the antibody concentration (µg/mL) of the anti-CS or anti-CUB autoantibodies after purification using CS- or CUB-affinity chromatography, respectively. Antibody concentrations were measured via spectrophotometry at optical density 280 nm (OD280 nm). Green or purple boxes indicate that autoantibodies were eluted from the CS- or CUB-affinity column, respectively, and that they consisted of anti-CS or anti-CUB autoantibodies because they showed specific binding to CS or CUB, respectively, in ELISA (Figure 1A-B), with the exception of the anti-CUB autoantibody fraction of patient F (*), which also recognized T2-T8 (Figure 1B). This anti-CUB autoantibody fraction was not studied further. Gray boxes indicate that no autoantibody was eluted from the CS- or CUB-affinity column (Figure 1A-B).