αIIb subunit peptides analyzed to determine the disulfide bond redox state
| Disulfide bond . | Cysteine . | Peptide sequence* . | Peptide score . | Peptide error (ppm) . |
|---|---|---|---|---|
| C56-C65 | C56 | LCPWR | 196† | –0.5 |
| C65 | RAEGGQCPSLL | 39 | –5.8 | |
| RAEGGQCPSLLF | 43 | 1.7 | ||
| C107-C130 | C107 | SDVIVACAPW | 286† | –2.4 |
| C130 | TEEAEKTPVGSCF | 68 | –3.5 | |
| EKTEEAEKTPVGSCF | 74 | 1.9 | ||
| TPVGSCF | 429† | –2.6 | ||
| C146-C167 | C146 | AEYSPCR | 38 | –3.4 |
| C167 | CEAGF | 433† | –2.9 | |
| C473-C484 | C473 | SCVLPQTK | 257† | –1.5 |
| C484 | TPVSCF | 432† | 4.4 | |
| TKTPVSCF | 451† | –1.2 | ||
| C490-C545 | C490 | NIQMCVGATGH | 379† | –2.6 |
| NIQM(oxidation)CVGATGH | 335† | –2.6 | ||
| C545 | HSPICHTTM | 40 | –2.1 | |
| HSPICHTTM(oxidation) | 284† | –0.3 | ||
| HSPICH | 410† | –2.0 | ||
| C602-C608 | C602, C608 | DCGEDDVCVPQLQL | 247† | –2.2 |
| C674-C687 | C674 | ICNQK | 375† | –3.3 |
| LICNQK | 284† | –2.5 | ||
| C687 | CELGNPM(oxidation)K | 341† | –2.5 | |
| CELGNPM(oxidation)KK | 41 | –2.4 | ||
| C826-C890 | C826 | QCFPQPPVNPL | 447† | 0.1 |
| C890 | CDLQEM(oxidation)AR | 286† | 0.8 |
| Disulfide bond . | Cysteine . | Peptide sequence* . | Peptide score . | Peptide error (ppm) . |
|---|---|---|---|---|
| C56-C65 | C56 | LCPWR | 196† | –0.5 |
| C65 | RAEGGQCPSLL | 39 | –5.8 | |
| RAEGGQCPSLLF | 43 | 1.7 | ||
| C107-C130 | C107 | SDVIVACAPW | 286† | –2.4 |
| C130 | TEEAEKTPVGSCF | 68 | –3.5 | |
| EKTEEAEKTPVGSCF | 74 | 1.9 | ||
| TPVGSCF | 429† | –2.6 | ||
| C146-C167 | C146 | AEYSPCR | 38 | –3.4 |
| C167 | CEAGF | 433† | –2.9 | |
| C473-C484 | C473 | SCVLPQTK | 257† | –1.5 |
| C484 | TPVSCF | 432† | 4.4 | |
| TKTPVSCF | 451† | –1.2 | ||
| C490-C545 | C490 | NIQMCVGATGH | 379† | –2.6 |
| NIQM(oxidation)CVGATGH | 335† | –2.6 | ||
| C545 | HSPICHTTM | 40 | –2.1 | |
| HSPICHTTM(oxidation) | 284† | –0.3 | ||
| HSPICH | 410† | –2.0 | ||
| C602-C608 | C602, C608 | DCGEDDVCVPQLQL | 247† | –2.2 |
| C674-C687 | C674 | ICNQK | 375† | –3.3 |
| LICNQK | 284† | –2.5 | ||
| C687 | CELGNPM(oxidation)K | 341† | –2.5 | |
| CELGNPM(oxidation)KK | 41 | –2.4 | ||
| C826-C890 | C826 | QCFPQPPVNPL | 447† | 0.1 |
| C890 | CDLQEM(oxidation)AR | 286† | 0.8 |
Peptides were detected by using both Byonic and Mascot analysis software, confirmed by using tandem mass spectrometry, and have errors <6 ppm. Only peptides with peak areas >3 million and contributing >10% of the total peptide peak areas for a given Cys were included in the analysis.
Cys (underlined) was labeled with 12C-iodoacetanilide or 13C-iodoacetanilide.
Score and error were determined by using Byonic software using αIIb integrin as reference.