Baseline characteristics and assessments of coagulation and organ failure in an experimental trauma transfusion model
| Parameter . | Crystalloid . | Crystalloid + rhADAMTS13 . | Plasma . | |||
|---|---|---|---|---|---|---|
| Before injury . | After 6 h . | Before injury . | After 6 h . | Before injury . | After 6 h . | |
| Weight (g) | 348 (336-369) | ND | 353 (348-379) | ND | 350 (336-380) | ND |
| Arterial blood gas | ||||||
| pH | 7.41 (7.40-7.46) | 7.33 (7.29-7.46) | 7.43 (7.37-7.48) | 7.35 (7.30-7.39) | 7.45 (7.40-7.46) | 7.33 (7.28-7.39) |
| pCO2 (mm Hg) | 34.9 (32.1-41.1) | 22.7 (19.7-31.0) | 34.6 (32.7-44.1) | 31.0 (28.8-33.7)* | 35.1 (31.9-37.5) | 26.6 (24.9-31.5) |
| HCO3− (mmol/L) | 23.5 (21.1-24.9) | 13.2 (11.8-14.9) | 23.6 (22.5-25.0) | 17.6 (16.1-18.2) *,** | 22.5 (21.6-24.0) | 14.1 (11.1-16.2) |
| BE (mEq/L) | −0.1 (−1.9 to 0.4) | −10.3 (−12.8-−9.3) | −0.5 (−0.9 to 0.6) | −7.6 (−8.3 to −5.8) *,** | −1.3 (−1.8 to 0.2) | −10.0 (−14.1 to −8.6) |
| Ca2+ (mmol/L) | 1.1 (1.1-1.1) | 0.9 (0.8-1.1) | 1.1 (1.0-1.2) | 1.0 (1.0-1.1) | 1.1 (1.0-1.1) | 0.9 (0.9-1.1) |
| Hematology | ||||||
| Hb (g/dL) | 15.1 (14.5-15.8) | 9.2 (8.1-10.2) | 15.3 (15.1-16.0) | 9.7 (9.2-10.0) | 15.3 (14.7-15.6) | 9.3 (8.6-9.6) |
| Hematocrit (%) | 44 (43-46) | 27 (26-28) | 46 (43-49) | 29 (27-29) | 45 (44-46) | 27 (26-28) |
| Leukocytes (×109/L) | 5.7 (4.7-6.4) | 3.3 (2.8-5.4) | 6.0 (5.4-6.7) | 5.4 (2.9-6.0) | 5.0 (4.3-6.7) | 4.4 (3.2-5.1) |
| Hepatic | ||||||
| AST (U/L) | 60 (58-69) | 6147 (4019-8179) | 63 (59-67) | 833 (362-1123) *,** | 64 (60-74) | 3354 (2277-5171) |
| ALT (U/L) | 51 (49-56) | 3334 (2307-5680) | 54 (49-57) | 365 (183-553) *,** | 53 (45-58) | 1996 (1451-4609) |
| Renal | ||||||
| Creatinine (μmol/L) | 25 (25-26) | 157 (154-168) | 25 (23-25) | 108 (98-122)*,** | 26 (25-27) | 161 (140-167) |
| Urine protein (g/L) | 0.8 (0.6-1.2) | 2.2 (1.6-2.8) | 0.8 (0.3-1.0)** | 1.9 (1.3-2.0) | 1.3 (0.8-1.6) | 2.1 (1.7-2.6) |
| Coagulation | ||||||
| EXTEM CT (s) | 50 (49-53) | 62 (53-89) | 53 (49-56) | 53 (47-58) | 49 (48-52) | 54 (47-59) |
| EXTEM CFt (s) | 41 (39-44) | 73 (56-122) | 44 (43-49) | 50 (45-58)* | 40 (39-43) | 52 (42-59)* |
| EXTEM MCF (mm) | 71 (70-72) | 60 (51-66) | 70 (69-70) | 67 (64-68) | 71 (69-72) | 67 (64-69) |
| EXTEM Li30 (%) | 100 (100-100) | 100 (100-100) | 100 (100-100) | 100 (100-100) | 100 (100-100) | 100 (100-100) |
| FIBTEM CT (s) | 47 (42-51) | 64 (51-299) | 46 (43-52) | 45 (43-53)* | 48 (45-51) | 47 (45-55) |
| FIBTEM MCF (mm) | 15 (14-17) | 6.5 (3.0-8.3) | 14.5 (13.3-20.5) | 8.0 (6.0-9.0) | 15.0 (14.0-16.5) | 11.0 (10.0-14.0)*,*** |
| Parameter . | Crystalloid . | Crystalloid + rhADAMTS13 . | Plasma . | |||
|---|---|---|---|---|---|---|
| Before injury . | After 6 h . | Before injury . | After 6 h . | Before injury . | After 6 h . | |
| Weight (g) | 348 (336-369) | ND | 353 (348-379) | ND | 350 (336-380) | ND |
| Arterial blood gas | ||||||
| pH | 7.41 (7.40-7.46) | 7.33 (7.29-7.46) | 7.43 (7.37-7.48) | 7.35 (7.30-7.39) | 7.45 (7.40-7.46) | 7.33 (7.28-7.39) |
| pCO2 (mm Hg) | 34.9 (32.1-41.1) | 22.7 (19.7-31.0) | 34.6 (32.7-44.1) | 31.0 (28.8-33.7)* | 35.1 (31.9-37.5) | 26.6 (24.9-31.5) |
| HCO3− (mmol/L) | 23.5 (21.1-24.9) | 13.2 (11.8-14.9) | 23.6 (22.5-25.0) | 17.6 (16.1-18.2) *,** | 22.5 (21.6-24.0) | 14.1 (11.1-16.2) |
| BE (mEq/L) | −0.1 (−1.9 to 0.4) | −10.3 (−12.8-−9.3) | −0.5 (−0.9 to 0.6) | −7.6 (−8.3 to −5.8) *,** | −1.3 (−1.8 to 0.2) | −10.0 (−14.1 to −8.6) |
| Ca2+ (mmol/L) | 1.1 (1.1-1.1) | 0.9 (0.8-1.1) | 1.1 (1.0-1.2) | 1.0 (1.0-1.1) | 1.1 (1.0-1.1) | 0.9 (0.9-1.1) |
| Hematology | ||||||
| Hb (g/dL) | 15.1 (14.5-15.8) | 9.2 (8.1-10.2) | 15.3 (15.1-16.0) | 9.7 (9.2-10.0) | 15.3 (14.7-15.6) | 9.3 (8.6-9.6) |
| Hematocrit (%) | 44 (43-46) | 27 (26-28) | 46 (43-49) | 29 (27-29) | 45 (44-46) | 27 (26-28) |
| Leukocytes (×109/L) | 5.7 (4.7-6.4) | 3.3 (2.8-5.4) | 6.0 (5.4-6.7) | 5.4 (2.9-6.0) | 5.0 (4.3-6.7) | 4.4 (3.2-5.1) |
| Hepatic | ||||||
| AST (U/L) | 60 (58-69) | 6147 (4019-8179) | 63 (59-67) | 833 (362-1123) *,** | 64 (60-74) | 3354 (2277-5171) |
| ALT (U/L) | 51 (49-56) | 3334 (2307-5680) | 54 (49-57) | 365 (183-553) *,** | 53 (45-58) | 1996 (1451-4609) |
| Renal | ||||||
| Creatinine (μmol/L) | 25 (25-26) | 157 (154-168) | 25 (23-25) | 108 (98-122)*,** | 26 (25-27) | 161 (140-167) |
| Urine protein (g/L) | 0.8 (0.6-1.2) | 2.2 (1.6-2.8) | 0.8 (0.3-1.0)** | 1.9 (1.3-2.0) | 1.3 (0.8-1.6) | 2.1 (1.7-2.6) |
| Coagulation | ||||||
| EXTEM CT (s) | 50 (49-53) | 62 (53-89) | 53 (49-56) | 53 (47-58) | 49 (48-52) | 54 (47-59) |
| EXTEM CFt (s) | 41 (39-44) | 73 (56-122) | 44 (43-49) | 50 (45-58)* | 40 (39-43) | 52 (42-59)* |
| EXTEM MCF (mm) | 71 (70-72) | 60 (51-66) | 70 (69-70) | 67 (64-68) | 71 (69-72) | 67 (64-69) |
| EXTEM Li30 (%) | 100 (100-100) | 100 (100-100) | 100 (100-100) | 100 (100-100) | 100 (100-100) | 100 (100-100) |
| FIBTEM CT (s) | 47 (42-51) | 64 (51-299) | 46 (43-52) | 45 (43-53)* | 48 (45-51) | 47 (45-55) |
| FIBTEM MCF (mm) | 15 (14-17) | 6.5 (3.0-8.3) | 14.5 (13.3-20.5) | 8.0 (6.0-9.0) | 15.0 (14.0-16.5) | 11.0 (10.0-14.0)*,*** |
Data are presented as median (IQR). Before trauma and before termination, samples were measured for different parameters.
ALT, alanine aminotransferase; AST, aspartate aminotransferase; BE, base excess; CFt, clot formation time; CT, clotting time; Hb, hemoglobin; Li30, lysis index, 30 minutes; MCF, maximum clot firmness; pCO2, partial arterial carbon dioxide pressure.
Bonferroni-adjusted *P < .05 compared with crystalloid, **P < .05 compared with plasma, and ***P < .05 compared with crystalloid + rhADAMTS13.