AAV vector quality attributes
| AAV sample . | qPCR titer, vg/mL . | Total AAV concentration, Cp/mL . | Capsid occupancy SEC-MALS, vg/Cp . | Capsid occupancy qPCR, vg/Cp . |
|---|---|---|---|---|
| hFIX-Padua | 7.74 × 1013 | 2.1 ± 0.1 × 1013 | 0.86 ± 0.05 | 3.67 |
| An96 | 2.52 × 1013 | 7.5 ± 0.5 × 1012 | 0.86 ± 0.03 | 3.36 |
| An96-Padua | 1.15 × 1013 | 3.4 ± 0.3 × 1012 | 0.99 ± 0.34 | 3.41 |
| AAV sample . | qPCR titer, vg/mL . | Total AAV concentration, Cp/mL . | Capsid occupancy SEC-MALS, vg/Cp . | Capsid occupancy qPCR, vg/Cp . |
|---|---|---|---|---|
| hFIX-Padua | 7.74 × 1013 | 2.1 ± 0.1 × 1013 | 0.86 ± 0.05 | 3.67 |
| An96 | 2.52 × 1013 | 7.5 ± 0.5 × 1012 | 0.86 ± 0.03 | 3.36 |
| An96-Padua | 1.15 × 1013 | 3.4 ± 0.3 × 1012 | 0.99 ± 0.34 | 3.41 |
Values are mean and standard deviation of 2 injections except An96-Padua (n = 1 because of lower titer and limited sample availability).
Cp, capsid particle.