Diagnostic performance of FCM and molecular profiles for NK-cell clonality assessment and the clonality score
| . | . | . | . | . | Univariate analysis . | Multivariate analysis . | . | ||
|---|---|---|---|---|---|---|---|---|---|
| Training set (n = 48) . | Se, % . | Sp, % . | PPV, % . | NPV, % . | Khi2 . | P . | Khi2(LR) . | P . | Score . |
| NK cell count >1 × 109/L | 69.6 | 84.0 | 80.0 | 75.0 | 12.023 | <.001 | 6.581 | .010 | 2 |
| Flow cytometry | |||||||||
| KIR-restricted phenotype* | 82.6 | 92.0 | 90.5 | 85.2 | 24.149 | <.001 | 9.077 | .003 | 2 |
| CD94 or NKG2Ahi† | 69.6 | 76.0 | 72.7 | 73.1 | 8.267 | .004 | 1.125 | .289 | 1 |
| STAT3, STAT5B, TET2 or TNFAIP3 status | |||||||||
| Mutated | 60.9 | 96.0 | 93.3 | 72.7 | 15.483 | <.001 | 5.222 | .022 | 2 |
| . | . | . | . | . | Univariate analysis . | Multivariate analysis . | . | ||
|---|---|---|---|---|---|---|---|---|---|
| Training set (n = 48) . | Se, % . | Sp, % . | PPV, % . | NPV, % . | Khi2 . | P . | Khi2(LR) . | P . | Score . |
| NK cell count >1 × 109/L | 69.6 | 84.0 | 80.0 | 75.0 | 12.023 | <.001 | 6.581 | .010 | 2 |
| Flow cytometry | |||||||||
| KIR-restricted phenotype* | 82.6 | 92.0 | 90.5 | 85.2 | 24.149 | <.001 | 9.077 | .003 | 2 |
| CD94 or NKG2Ahi† | 69.6 | 76.0 | 72.7 | 73.1 | 8.267 | .004 | 1.125 | .289 | 1 |
| STAT3, STAT5B, TET2 or TNFAIP3 status | |||||||||
| Mutated | 60.9 | 96.0 | 93.3 | 72.7 | 15.483 | <.001 | 5.222 | .022 | 2 |
| NK-cell clonality score ≥4 . | Se, % . | Sp, % . | PPV, % . | NPV, % . | AUC . | P . | . | . | . |
|---|---|---|---|---|---|---|---|---|---|
| Training set (n = 48) | 82.6 | 96.0 | 95.0 | 95.7 | 0.937 | <.001 | |||
| Validation set (n = 38) | 83.3 | 100.0 | 100.0 | 87.0 | 0.946 | <.001 |
| NK-cell clonality score ≥4 . | Se, % . | Sp, % . | PPV, % . | NPV, % . | AUC . | P . | . | . | . |
|---|---|---|---|---|---|---|---|---|---|
| Training set (n = 48) | 82.6 | 96.0 | 95.0 | 95.7 | 0.937 | <.001 | |||
| Validation set (n = 38) | 83.3 | 100.0 | 100.0 | 87.0 | 0.946 | <.001 |
AUC, ROC area under the curve; LR, logistical regression; NPV, negative predictive value; PPV, positive predictive value; Se, sensitivity; Sp, specificity.
A KIR-restricted phenotype was defined as the loss of at ≥2 KIRs (CD158A <12%, CD158B <9%, or NKB1 <4% of NK cells).
CD94 or NKG2A expressed by >77% of NK cells.