Anti-platelet and anti-RBC antibodies used to mediate phagocytosis
| Antibody . | Species . | Subclass . | Activating FcγR binding . | Target cell . | Target antigen . |
|---|---|---|---|---|---|
| MWReg30* | Rat | IgG1 | FcγRIII14 | Platelet | GPIIb |
| 5C4* | Mouse | IgG2a | FcγRI, III, IV27 | Platelet | GPIIb |
| 2G1 | Mouse | IgG2a | FcγRI, III, IV27 | Platelet | GPIIb |
| 10B5 | Mouse | IgG2b | FcγRIII†/FcγRI, III, IV27 | Platelet | GPIIb |
| TER-119 (WT) | Rat | IgG2b | FcγRI, III, IV28 | RBC | Glycophorin A–associated protein (Ly76) |
| TER-119-ms IgG1 | Switched to mouse IgG1 | IgG1 | FcγRIII27 | RBC | Glycophorin A–associated protein (Ly76) |
| TER-119-ms IgG2a | Switched to mouse IgG2a | IgG2a | FcγRI, III, IV27 | RBC | Glycophorin A–associated protein (Ly76) |
| Antibody . | Species . | Subclass . | Activating FcγR binding . | Target cell . | Target antigen . |
|---|---|---|---|---|---|
| MWReg30* | Rat | IgG1 | FcγRIII14 | Platelet | GPIIb |
| 5C4* | Mouse | IgG2a | FcγRI, III, IV27 | Platelet | GPIIb |
| 2G1 | Mouse | IgG2a | FcγRI, III, IV27 | Platelet | GPIIb |
| 10B5 | Mouse | IgG2b | FcγRIII†/FcγRI, III, IV27 | Platelet | GPIIb |
| TER-119 (WT) | Rat | IgG2b | FcγRI, III, IV28 | RBC | Glycophorin A–associated protein (Ly76) |
| TER-119-ms IgG1 | Switched to mouse IgG1 | IgG1 | FcγRIII27 | RBC | Glycophorin A–associated protein (Ly76) |
| TER-119-ms IgG2a | Switched to mouse IgG2a | IgG2a | FcγRI, III, IV27 | RBC | Glycophorin A–associated protein (Ly76) |
ms IgG1, mouse IgG1; ms IgG2a, mouse IgG2a; WT, wild-type.
Antibodies were used to induce thrombocytopenia in vivo.
Clone 10B5 mediated FcγRIII-dependent activity in macrophage phagocytosis, although murine IgG2b is typically reported to bind all murine activating FcγRs27 ; clone 10B5 was confirmed as murine IgG2b, κ by mass spectrometry.