CAD: handling of samples
| Analysis . | Material . | Sampling . | Handling of sample . |
|---|---|---|---|
| Hb, blood cell counts | Blood | EDTA vacuum tube | Prewarm at 37-38°C before analysis if problems with agglutination |
| CA titer, thermal amplitude, immunoglobulin quantification, SPEP, immune fixation | Serum or plasma | Blood is drawn into prewarmed vacuum tubes (for serum, no gel or additive); place in warming cabinet or water bath at 37-38°C | Keep at 37-38°C until serum/plasma has been removed from clot/cells, after which sample can be handled at room temperature |
| Flow cytometry | Bone marrow aspirate (too low sensitivity if performed in peripheral blood) | Add EDTA or heparin | Prewarming before analysis will often be sufficient; if not, wash cells at 37-38°C; for description, see Ulvestad et al40 |
| Analysis . | Material . | Sampling . | Handling of sample . |
|---|---|---|---|
| Hb, blood cell counts | Blood | EDTA vacuum tube | Prewarm at 37-38°C before analysis if problems with agglutination |
| CA titer, thermal amplitude, immunoglobulin quantification, SPEP, immune fixation | Serum or plasma | Blood is drawn into prewarmed vacuum tubes (for serum, no gel or additive); place in warming cabinet or water bath at 37-38°C | Keep at 37-38°C until serum/plasma has been removed from clot/cells, after which sample can be handled at room temperature |
| Flow cytometry | Bone marrow aspirate (too low sensitivity if performed in peripheral blood) | Add EDTA or heparin | Prewarming before analysis will often be sufficient; if not, wash cells at 37-38°C; for description, see Ulvestad et al40 |
Reprinted with permission from Berentsen et al.41