Mechanisms of CD19 loss during blinatumomab treatment
| Sample ID . | CD19 LOH or CNA . | CD19 mutations . | Sum AF of CD19 mutations . | CD19 RNA expression (log CPM) . | Summary of CD19 loss . |
|---|---|---|---|---|---|
| SJBALL042232_R2 | Loss of chromosome 16 | p.Y259fs (c.775 T>TGT) | 0.79 | Normal (5.3) | CD19 mutation |
| p.Y259fs (c.776 A>ATTGGAGATCCC) | Loss of chromosome 16 | ||||
| SJALL061897_R1 | Loss of chromosome 16 | p.K258fs (c.773 AGTATTAT>AAAAAAA) | 0.79 | Normal (5.2) | CD19 mutation |
| Loss of chromosome 16 | |||||
| SJALL061895_R1 | Loss of chromosome 16 | p.109fs (c.326 C>CA) | 0.65 | Normal (5.4) | CD19 mutation |
| p.Q186sp | Loss of chromosome 16 | ||||
| p.P278sp | |||||
| SJALL061893_R1 | No | p.P60del (c.179 CCTT>C) | 0.73 | Normal (6.1) | CD19 mutation |
| Allele-specific expression | |||||
| SJALL061894_R1 | No | p.S269* (c.806 C>A) | 0.15 | Low (0.4) | CD19 mutation |
| Allele-specific expression (low) | |||||
| SJBALL042246_R2 | No | None | 0 | Low (0.8) | Low level of CD19 expression |
| SJALL061896_R1 | No | None | 0 | Normal (6.2) | CD19 wild type |
| CD81 mutation |
| Sample ID . | CD19 LOH or CNA . | CD19 mutations . | Sum AF of CD19 mutations . | CD19 RNA expression (log CPM) . | Summary of CD19 loss . |
|---|---|---|---|---|---|
| SJBALL042232_R2 | Loss of chromosome 16 | p.Y259fs (c.775 T>TGT) | 0.79 | Normal (5.3) | CD19 mutation |
| p.Y259fs (c.776 A>ATTGGAGATCCC) | Loss of chromosome 16 | ||||
| SJALL061897_R1 | Loss of chromosome 16 | p.K258fs (c.773 AGTATTAT>AAAAAAA) | 0.79 | Normal (5.2) | CD19 mutation |
| Loss of chromosome 16 | |||||
| SJALL061895_R1 | Loss of chromosome 16 | p.109fs (c.326 C>CA) | 0.65 | Normal (5.4) | CD19 mutation |
| p.Q186sp | Loss of chromosome 16 | ||||
| p.P278sp | |||||
| SJALL061893_R1 | No | p.P60del (c.179 CCTT>C) | 0.73 | Normal (6.1) | CD19 mutation |
| Allele-specific expression | |||||
| SJALL061894_R1 | No | p.S269* (c.806 C>A) | 0.15 | Low (0.4) | CD19 mutation |
| Allele-specific expression (low) | |||||
| SJBALL042246_R2 | No | None | 0 | Low (0.8) | Low level of CD19 expression |
| SJALL061896_R1 | No | None | 0 | Normal (6.2) | CD19 wild type |
| CD81 mutation |
CNA, copy-number abnormality; CPM, count per million; LOH, loss of heterozygosity.