Differently abundant proteins between neutrophils from patients with GPS and healthy controls
| Gene names . | logFC . | Adjusted P . | Direction . | Granule . |
|---|---|---|---|---|
| ADAM8 | −1.323657312 | 1.25871E-07 | Down | SG |
| AK1 | 1.404751471 | 0.006640861 | Up | |
| ALDH3B1 | −1.064129038 | 9,03216E-06 | Down | |
| ANTXR2 | −1.420443758 | 0.001203625 | Down | SV |
| AZU1 | −1.258930678 | 0.015537439 | Down | AG |
| C14orf142 | 1.944269282 | 0.038452512 | Up | |
| CD200R1 | −1.706012645 | 0.002598112 | Down | |
| CEACAM1 | −1.00298783 | 1.27051E-05 | Down | SG/GG |
| CD93 | −1.201001783 | 1.63897E-05 | Down | FG |
| CHIT1* | −2.580734627 | 0.004690497 | Down | SG |
| CIB1 | −1.553545064 | 2.0362E-10 | Down | SV |
| CLEC4D | −1.683458526 | 1.05203E-06 | Down | SG |
| CLEC5A | −1.438176815 | 0.001199003 | Down | SG |
| CRISP3 | −1,053756172 | 6.28887E-06 | Down | SG |
| CRISPLD2 | −3.028483512 | 1.30474E-06 | Down | FG |
| CTCF | 1.093635528 | 0.010377533 | Up | |
| DDI2 | 1.235789759 | 0.004754332 | Up | |
| FBXO7 | 1.86528072 | 0.018502334 | Up | |
| FCAR | −1.157873873 | 5.29839E-07 | Down | SG |
| FCN1 | −1.358795862 | 0.008014707 | Down | FG |
| FGL2 | −3.152449135 | 2.23273E-08 | Down | FG |
| FLNB | 1.591666804 | 0.028032967 | Up | |
| FPR2 | −3.215391757 | 2.87143E-07 | Down | FG |
| GP1BB | 2.489535838 | 0.002505912 | Up | |
| HBA1 | 1.425726403 | 0.036719775 | Up | |
| HBB | 1.499401526 | 0.024715387 | Up | † |
| HBD | 2.18089191 | 0.00805624 | Up | |
| IGHM | 4.564716704 | 0.001517623 | Up | |
| ISG15 | 1.50580967 | 0.013120869 | Up | |
| ITGA2B | 2.17313411 | 0.034495612 | Up | |
| ITGB3 | 3.256606076 | 0.000590977 | Up | |
| ITM2B | −1,776889478 | 0.005035806 | Down | SV |
| LCN2* | −1.067419243 | 9.36095E-07 | Down | SG |
| LILRA3 | −1.191339116 | 0.03617178 | Down | SG/GG |
| LONRF2 | −1.615776321 | 0.040658862 | Down | |
| LPCAT1 | −1.166004931 | 0.008161495 | Down | AG |
| LRG1 | −1.656966487 | 3.116E-08 | Down | SG |
| LST1 | −1.335978979 | 0.038055338 | Down | CM |
| LTB4R* | −1.144669658 | 0.001994964 | Down | SG |
| MCEMP1 | −1.726928642 | 1.71313E-09 | Down | SG |
| MGAM | −1.311041164 | 3.77199E-08 | Down | FG |
| MMP8* | −1.524649658 | 8.97816E-08 | Down | SG |
| MMP9* | −2.520262691 | 3.71309E-10 | Down | GG |
| MMP25 | −1.166679848 | 0.034288334 | Down | SV |
| NBEAL2 | −5.458711875 | 1.10983E-16 | Down | GG |
| NUDT3 | 1.047414287 | 0.018146866 | Up | |
| OLR1* | −1.275030481 | 9.03216E-06 | Down | SG |
| ORM2* | −1.92503296 | 0.003408997 | Down | SG |
| OSCAR* | −1.663676016 | 0.000146155 | Down | SG |
| PF4 | −3.245437395 | 3.87295E-05 | Down | † |
| PGLYRP1 | −1.730698451 | 1.1023E-10 | Down | SG |
| PPBP | −1.735672277 | 0.016495775 | Down | GG |
| PTPRJ | −1.009341488 | 5.32917E-09 | Down | SG |
| PTX3 | −1.237195701 | 8.33899E-07 | Down | SG |
| QPCT | −1.871870602 | 5.32917E-09 | Down | GG |
| QSOX1 | −1.319014529 | 1.3305E-08 | Down | SG |
| RNF123 | 2.052297543 | 0.000170734 | Up | |
| RPL37A | 1.619666522 | 7.9634E-07 | Up | |
| SDPR | 1.423250247 | 0.028032967 | Up | |
| SIRPB1 | −1,04135498 | 0.010205733 | Down | CM |
| SLC44A1 | −1,298388194 | 0.015698875 | Down | CM |
| SPAG9 | 1.612871903 | 0.01723901 | Up | |
| SPTB | 2.478165209 | 0.014456575 | Up | CM |
| SRGN* | −2.369263809 | 7.17865E-08 | Down | Preferential SG |
| TBC1D24 | 1.064763056 | 0.016157178 | Up | |
| THEMIS | 2.939359331 | 0.02286843 | Up | |
| TPM4 | 1.033728537 | 0.001199003 | Up | |
| TSPAN14* | −1.199040302 | 0.000158716 | Down | SG |
| TTN | 2.775421702 | 0.034495612 | Up | |
| TUBB1 | 2.386733997 | 0.020660207 | Up | |
| TXNDC17 | 1.464401309 | 0.022068896 | Up | |
| YOD1 | 1.670178267 | 0.002453601 | Up |
| Gene names . | logFC . | Adjusted P . | Direction . | Granule . |
|---|---|---|---|---|
| ADAM8 | −1.323657312 | 1.25871E-07 | Down | SG |
| AK1 | 1.404751471 | 0.006640861 | Up | |
| ALDH3B1 | −1.064129038 | 9,03216E-06 | Down | |
| ANTXR2 | −1.420443758 | 0.001203625 | Down | SV |
| AZU1 | −1.258930678 | 0.015537439 | Down | AG |
| C14orf142 | 1.944269282 | 0.038452512 | Up | |
| CD200R1 | −1.706012645 | 0.002598112 | Down | |
| CEACAM1 | −1.00298783 | 1.27051E-05 | Down | SG/GG |
| CD93 | −1.201001783 | 1.63897E-05 | Down | FG |
| CHIT1* | −2.580734627 | 0.004690497 | Down | SG |
| CIB1 | −1.553545064 | 2.0362E-10 | Down | SV |
| CLEC4D | −1.683458526 | 1.05203E-06 | Down | SG |
| CLEC5A | −1.438176815 | 0.001199003 | Down | SG |
| CRISP3 | −1,053756172 | 6.28887E-06 | Down | SG |
| CRISPLD2 | −3.028483512 | 1.30474E-06 | Down | FG |
| CTCF | 1.093635528 | 0.010377533 | Up | |
| DDI2 | 1.235789759 | 0.004754332 | Up | |
| FBXO7 | 1.86528072 | 0.018502334 | Up | |
| FCAR | −1.157873873 | 5.29839E-07 | Down | SG |
| FCN1 | −1.358795862 | 0.008014707 | Down | FG |
| FGL2 | −3.152449135 | 2.23273E-08 | Down | FG |
| FLNB | 1.591666804 | 0.028032967 | Up | |
| FPR2 | −3.215391757 | 2.87143E-07 | Down | FG |
| GP1BB | 2.489535838 | 0.002505912 | Up | |
| HBA1 | 1.425726403 | 0.036719775 | Up | |
| HBB | 1.499401526 | 0.024715387 | Up | † |
| HBD | 2.18089191 | 0.00805624 | Up | |
| IGHM | 4.564716704 | 0.001517623 | Up | |
| ISG15 | 1.50580967 | 0.013120869 | Up | |
| ITGA2B | 2.17313411 | 0.034495612 | Up | |
| ITGB3 | 3.256606076 | 0.000590977 | Up | |
| ITM2B | −1,776889478 | 0.005035806 | Down | SV |
| LCN2* | −1.067419243 | 9.36095E-07 | Down | SG |
| LILRA3 | −1.191339116 | 0.03617178 | Down | SG/GG |
| LONRF2 | −1.615776321 | 0.040658862 | Down | |
| LPCAT1 | −1.166004931 | 0.008161495 | Down | AG |
| LRG1 | −1.656966487 | 3.116E-08 | Down | SG |
| LST1 | −1.335978979 | 0.038055338 | Down | CM |
| LTB4R* | −1.144669658 | 0.001994964 | Down | SG |
| MCEMP1 | −1.726928642 | 1.71313E-09 | Down | SG |
| MGAM | −1.311041164 | 3.77199E-08 | Down | FG |
| MMP8* | −1.524649658 | 8.97816E-08 | Down | SG |
| MMP9* | −2.520262691 | 3.71309E-10 | Down | GG |
| MMP25 | −1.166679848 | 0.034288334 | Down | SV |
| NBEAL2 | −5.458711875 | 1.10983E-16 | Down | GG |
| NUDT3 | 1.047414287 | 0.018146866 | Up | |
| OLR1* | −1.275030481 | 9.03216E-06 | Down | SG |
| ORM2* | −1.92503296 | 0.003408997 | Down | SG |
| OSCAR* | −1.663676016 | 0.000146155 | Down | SG |
| PF4 | −3.245437395 | 3.87295E-05 | Down | † |
| PGLYRP1 | −1.730698451 | 1.1023E-10 | Down | SG |
| PPBP | −1.735672277 | 0.016495775 | Down | GG |
| PTPRJ | −1.009341488 | 5.32917E-09 | Down | SG |
| PTX3 | −1.237195701 | 8.33899E-07 | Down | SG |
| QPCT | −1.871870602 | 5.32917E-09 | Down | GG |
| QSOX1 | −1.319014529 | 1.3305E-08 | Down | SG |
| RNF123 | 2.052297543 | 0.000170734 | Up | |
| RPL37A | 1.619666522 | 7.9634E-07 | Up | |
| SDPR | 1.423250247 | 0.028032967 | Up | |
| SIRPB1 | −1,04135498 | 0.010205733 | Down | CM |
| SLC44A1 | −1,298388194 | 0.015698875 | Down | CM |
| SPAG9 | 1.612871903 | 0.01723901 | Up | |
| SPTB | 2.478165209 | 0.014456575 | Up | CM |
| SRGN* | −2.369263809 | 7.17865E-08 | Down | Preferential SG |
| TBC1D24 | 1.064763056 | 0.016157178 | Up | |
| THEMIS | 2.939359331 | 0.02286843 | Up | |
| TPM4 | 1.033728537 | 0.001199003 | Up | |
| TSPAN14* | −1.199040302 | 0.000158716 | Down | SG |
| TTN | 2.775421702 | 0.034495612 | Up | |
| TUBB1 | 2.386733997 | 0.020660207 | Up | |
| TXNDC17 | 1.464401309 | 0.022068896 | Up | |
| YOD1 | 1.670178267 | 0.002453601 | Up |
Results of the comparison between GPS (n = 11) and control (n = 15) neutrophils, as represented in the volcano plot of Figure 2A. Shown are the proteins more (up direction) or less (down direction) abundant in GPS neutrophils compared to healthy control neutrophils, with a restriction of a twofold increase or decrease in abundance. Granule annotation was defined according to Rorvig et al25 and adjudicated using cluster enrichment from Hoogendijk et al.21
CM, cell membrane; FG, ficolin-1-rich granule; logFC, log fold change; SV, secretory vesicle.
Deficient in SGD neutrophil proteomics according to Serwas et al.40
Possible contamination.