Table 1. Molecular and IHC data for... | American Society of Hematology

Table 1.

Molecular and IHC data for NOTCH2-mutated DLBCL cases

DLBCL case ID	Notch2 sequence variant²⁹	Variant reads/total (%)	LymphGen subtype³⁴	Cell of origin³²	BCL6 translocation	BCL6 IHC*	NICD2 IHC, % POS cells*	Total Notch2 IHC, % POS cells*
DLC_0048	p.H2293Efs*21	38/289 (13)	EZB/A53	UNCL	NEG	POS (strong)	0	0
DLC_0060	p.R2400*	9/25 (36)	Other	GCB	POS	POS (strong)	0	0
DLC_0201	p.Q2367*	69/173 (40)	BN2	ABC	POS	POS (weak, subset)	0	0
DLC_0231	p.Q2361*	6/21 (29)	BN2/A53	ABC	POS	POS (strong)	0	10
DLC_0239	p.I2304Hfs*9	292/400 (73)	Other	UNCL	NEG	NEG (weak, subset)	0	30
DLC_0242	p.T2355Hfs*45	2/4 (50)	ST2	GCB	NEG	POS (strong)	0	0
DLC_0269	p.Y2383*	93/194 (48)	BN2	GCB	POS	POS (strong)	0	30
DLC_0319	p.E2290Kfs*5	10/26 (38)	A53	ABC	NEG	POS (weak, subset)	0	0
DLC_0330	p.R2400*	9/46 (20)	MCD	ABC	POS	POS (strong)	0	0

DLBCL case ID	Notch2 sequence variant²⁹	Variant reads/total (%)	LymphGen subtype³⁴	Cell of origin³²	BCL6 translocation	BCL6 IHC*	NICD2 IHC, % POS cells*	Total Notch2 IHC, % POS cells*
DLC_0048	p.H2293Efs*21	38/289 (13)	EZB/A53	UNCL	NEG	POS (strong)	0	0
DLC_0060	p.R2400*	9/25 (36)	Other	GCB	POS	POS (strong)	0	0
DLC_0201	p.Q2367*	69/173 (40)	BN2	ABC	POS	POS (weak, subset)	0	0
DLC_0231	p.Q2361*	6/21 (29)	BN2/A53	ABC	POS	POS (strong)	0	10
DLC_0239	p.I2304Hfs*9	292/400 (73)	Other	UNCL	NEG	NEG (weak, subset)	0	30
DLC_0242	p.T2355Hfs*45	2/4 (50)	ST2	GCB	NEG	POS (strong)	0	0
DLC_0269	p.Y2383*	93/194 (48)	BN2	GCB	POS	POS (strong)	0	30
DLC_0319	p.E2290Kfs*5	10/26 (38)	A53	ABC	NEG	POS (weak, subset)	0	0
DLC_0330	p.R2400*	9/46 (20)	MCD	ABC	POS	POS (strong)	0	0

ABC, activated B cell; GCB, germinal center B; ID, identifier; NEG, negative; POS, positive; UNCL, unclassifiable.

See supplemental Figure 4 for representative staining results.