Immune checkpoint expression in TP53 mutant vs wildtype (WT) patients
| . | TP53 WT . | TP53 mutant . | P* . |
|---|---|---|---|
| Stem cells (CD3−, CD11b−, CD14−, CD34+, CD38−) | |||
| PD-1 | 1.33 (0-30.6) | 0.14 (0-5.79) | <.0001 |
| PDL1 | 10.9 (0.63-60.8) | 16.3 (1.92-71.7) | .0092 |
| PDL2 | 0.3 (0-14.6) | 0.094 (0-40.5) | .4005 |
| TIM-3 | 1.3 (0-34.7) | 3.86 (0.24-5.92) | .3902 |
| Progenitor cells (CD3−, CD11b−, CD14−, CD34+, CD38+) | |||
| PD-1 | 1.29 (0.039-13.1) | 0.32 (0-4.21) | <.0001 |
| PDL1 | 10.2 (0.25-56.5) | 20.7 (0.31-64.7) | .3350 |
| PDL2 | 0.91 (0.065-12.3) | 0.45 (0.11-3.06) | .0010 |
| TIM-3 | 3.64 (0.46-46.2) | 6.97 (0.89-7.69) | .6059 |
| G-MDSCs (CD3−, CD33+, CD38−, CD11b+, CD14−) | |||
| PD-1 | 24.75 (0.13-82.5) | 16.9 (0.5-59.9) | .0015 |
| PDL1 | 76.1 (0.35-98.7) | 77.6 (20.3-97.6) | .5100 |
| PDL2 | 0.42 (0-8.9) | 0.74 (0-4.64) | .1908 |
| TIM-3 | 9.8 (1.42-46.6) | 13.65 (5.72-43.5) | .3272 |
| Erythroid cells (CD3−, CD71+) | |||
| PD-1 | 27.9 (3.64-67.8) | 8.66 (0.09-41.4) | <.0001 |
| PDL1 | 92.6 (5.85-99.5) | 93.2 (24.2-99.5) | .6328 |
| PDL2 | 0.96 (0.15-11.5) | 1.02 (0.079-5.23) | .8005 |
| TIM-3 | 8.69 (0.95-44.1) | 8.26 (2.23-24.9) | .7046 |
| Myeloid cells (CD33+) | |||
| PD-1 | 7.78 (0.18-52.5) | 4.8 (0.33-27.2) | .0035 |
| PDL1 | 18.8 (0.6-92.3) | 31.3 (1.53-64.7) | .0874 |
| PDL2 | 2.56 (0.36-15.2) | 1.85 (0.18-8.8) | .0848 |
| TIM-3 | 11.5 (0.99-70.9) | 10.2 (5.28-21.9) | .4344 |
| All T cells (CD3+) | |||
| PD-1 | 5.465 (1.37-43) | 4.485 (0.64-21.1) | .0645 |
| TIM-3 | 1.74 (0.17-15.8) | 1.9 (0.35-9.72) | .6191 |
| LAG-3 | 0.51 (0.037-13.3) | 0.315 (0.025-2.54) | .0093 |
| OX-40 | 7.195 (1.11-28.5) | 3.2 (0.95-30.8) | <.0001 |
| CTLA-4 | 0.235 (0.043-5.22) | 0.13 (0.027-1.74) | .0004 |
| 4-1BB | 12.2 (1.28-39.1) | 13.1 (0.26-63.3) | .99 |
| ICOS | 7.15 (0.29-34.4) | 12.7 (0.77-42.3) | .0097 |
| Cytotoxic T cells (CD3+, CD8+) | |||
| PD-1 | 26.9 (10.1-59.6) | 24.15 (2.14-49) | .0754 |
| TIM-3 | 1.59 (0.091-16.8) | 1.55 (0.12-9.34) | .5061 |
| LAG-3 | 0.485 (0.061-9.39) | 0.245 (0-7.01) | .0045 |
| OX-40 | 6.435 (1.27-30.2) | 2.34 (0.54-14) | <.0001 |
| CTLA-4 | 0.105 (1-6.43) | 0.0445 (0-0.7) | .0157 |
| 4-1BB | 19.65 (2.2-85.8) | 37.85 (0.62-77.5) | .1546 |
| ICOS | 1.43 (0.18-30.9) | 4.93 (0.046-28) | .0008 |
| Helper T cells (CD3+, CD4+) | |||
| PD-1 | 4.33 (0.78-42.1) | 3.725 (0.49-15.9) | .0647 |
| TIM-3 | 2.12 (0.18-22.1) | 2.15 (0.45-10.5) | .5358 |
| LAG-3 | 0.695 (0.036-18.4) | 0.39 (0.029-2.91) | .0167 |
| OX-40 | 7.675 (1.04-35.2) | 3.75 (1.23-35.1) | .0002 |
| CTLA-4 | 0.225 (0.025-5.62) | 0.13 (0.026-1.71) | .0028 |
| 4-1BB | 7.675 (0.73-31) | 5.475 (0.2-67.2) | .1621 |
| ICOS | 11.3 (0.84-45.7) | 18.05 (1.5-57) | .0131 |
| Treg cells (CD3+, CD4+, CD25+, CD127low/−) | |||
| PD-1 | 5.195 (0.89-72.5) | 3.58 (0.4-17.6) | .0178 |
| TIM-3 | 4.72 (0.28-70.9) | 2.85 (1.9-11.6) | .2035 |
| LAG-3 | 2.425 (0.069-62.3) | 1.865 (0.049-16.7) | .0965 |
| OX-40 | 10.4 (1.54-46.2) | 5.82 (2.17-39.6) | .0128 |
| CTLA-4 | 1.3 (0.14-18.2) | 0.75 (0.16-9.35) | .0702 |
| 4-1BB | 10.85 (1.95-70.9) | 6.01 (1.29-29.4) | .0038 |
| ICOS | 14.45 (1.41-68.4) | 16.85 (2.17-48.4) | .0854 |
| NK cells (CD3−, CD56+) | |||
| PD-1 | 3.69 (0.43-36.2) | 1.94 (0.098-22.5) | .0072 |
| TIM-3 | 4.11 (0-38.5) | 4.31 (2.13-18.4) | .6334 |
| LAG-3 | 1.58 (0-27.5) | 0.765 (0-29.8) | .0038 |
| OX-40 | 15.3 (1.23-77.5) | 4.1 (0.28-41.9) | <.0001 |
| CTLA-4 | 0.54 (0.016-18.6) | 0.25 (1-4.08) | .0298 |
| 4-1BB | 9.95 (1.24-47) | 5.005 (0.36-33) | .0023 |
| ICOS | 0.57 (0-8.35) | 0.52 (0.13-3.63) | .5216 |
| . | TP53 WT . | TP53 mutant . | P* . |
|---|---|---|---|
| Stem cells (CD3−, CD11b−, CD14−, CD34+, CD38−) | |||
| PD-1 | 1.33 (0-30.6) | 0.14 (0-5.79) | <.0001 |
| PDL1 | 10.9 (0.63-60.8) | 16.3 (1.92-71.7) | .0092 |
| PDL2 | 0.3 (0-14.6) | 0.094 (0-40.5) | .4005 |
| TIM-3 | 1.3 (0-34.7) | 3.86 (0.24-5.92) | .3902 |
| Progenitor cells (CD3−, CD11b−, CD14−, CD34+, CD38+) | |||
| PD-1 | 1.29 (0.039-13.1) | 0.32 (0-4.21) | <.0001 |
| PDL1 | 10.2 (0.25-56.5) | 20.7 (0.31-64.7) | .3350 |
| PDL2 | 0.91 (0.065-12.3) | 0.45 (0.11-3.06) | .0010 |
| TIM-3 | 3.64 (0.46-46.2) | 6.97 (0.89-7.69) | .6059 |
| G-MDSCs (CD3−, CD33+, CD38−, CD11b+, CD14−) | |||
| PD-1 | 24.75 (0.13-82.5) | 16.9 (0.5-59.9) | .0015 |
| PDL1 | 76.1 (0.35-98.7) | 77.6 (20.3-97.6) | .5100 |
| PDL2 | 0.42 (0-8.9) | 0.74 (0-4.64) | .1908 |
| TIM-3 | 9.8 (1.42-46.6) | 13.65 (5.72-43.5) | .3272 |
| Erythroid cells (CD3−, CD71+) | |||
| PD-1 | 27.9 (3.64-67.8) | 8.66 (0.09-41.4) | <.0001 |
| PDL1 | 92.6 (5.85-99.5) | 93.2 (24.2-99.5) | .6328 |
| PDL2 | 0.96 (0.15-11.5) | 1.02 (0.079-5.23) | .8005 |
| TIM-3 | 8.69 (0.95-44.1) | 8.26 (2.23-24.9) | .7046 |
| Myeloid cells (CD33+) | |||
| PD-1 | 7.78 (0.18-52.5) | 4.8 (0.33-27.2) | .0035 |
| PDL1 | 18.8 (0.6-92.3) | 31.3 (1.53-64.7) | .0874 |
| PDL2 | 2.56 (0.36-15.2) | 1.85 (0.18-8.8) | .0848 |
| TIM-3 | 11.5 (0.99-70.9) | 10.2 (5.28-21.9) | .4344 |
| All T cells (CD3+) | |||
| PD-1 | 5.465 (1.37-43) | 4.485 (0.64-21.1) | .0645 |
| TIM-3 | 1.74 (0.17-15.8) | 1.9 (0.35-9.72) | .6191 |
| LAG-3 | 0.51 (0.037-13.3) | 0.315 (0.025-2.54) | .0093 |
| OX-40 | 7.195 (1.11-28.5) | 3.2 (0.95-30.8) | <.0001 |
| CTLA-4 | 0.235 (0.043-5.22) | 0.13 (0.027-1.74) | .0004 |
| 4-1BB | 12.2 (1.28-39.1) | 13.1 (0.26-63.3) | .99 |
| ICOS | 7.15 (0.29-34.4) | 12.7 (0.77-42.3) | .0097 |
| Cytotoxic T cells (CD3+, CD8+) | |||
| PD-1 | 26.9 (10.1-59.6) | 24.15 (2.14-49) | .0754 |
| TIM-3 | 1.59 (0.091-16.8) | 1.55 (0.12-9.34) | .5061 |
| LAG-3 | 0.485 (0.061-9.39) | 0.245 (0-7.01) | .0045 |
| OX-40 | 6.435 (1.27-30.2) | 2.34 (0.54-14) | <.0001 |
| CTLA-4 | 0.105 (1-6.43) | 0.0445 (0-0.7) | .0157 |
| 4-1BB | 19.65 (2.2-85.8) | 37.85 (0.62-77.5) | .1546 |
| ICOS | 1.43 (0.18-30.9) | 4.93 (0.046-28) | .0008 |
| Helper T cells (CD3+, CD4+) | |||
| PD-1 | 4.33 (0.78-42.1) | 3.725 (0.49-15.9) | .0647 |
| TIM-3 | 2.12 (0.18-22.1) | 2.15 (0.45-10.5) | .5358 |
| LAG-3 | 0.695 (0.036-18.4) | 0.39 (0.029-2.91) | .0167 |
| OX-40 | 7.675 (1.04-35.2) | 3.75 (1.23-35.1) | .0002 |
| CTLA-4 | 0.225 (0.025-5.62) | 0.13 (0.026-1.71) | .0028 |
| 4-1BB | 7.675 (0.73-31) | 5.475 (0.2-67.2) | .1621 |
| ICOS | 11.3 (0.84-45.7) | 18.05 (1.5-57) | .0131 |
| Treg cells (CD3+, CD4+, CD25+, CD127low/−) | |||
| PD-1 | 5.195 (0.89-72.5) | 3.58 (0.4-17.6) | .0178 |
| TIM-3 | 4.72 (0.28-70.9) | 2.85 (1.9-11.6) | .2035 |
| LAG-3 | 2.425 (0.069-62.3) | 1.865 (0.049-16.7) | .0965 |
| OX-40 | 10.4 (1.54-46.2) | 5.82 (2.17-39.6) | .0128 |
| CTLA-4 | 1.3 (0.14-18.2) | 0.75 (0.16-9.35) | .0702 |
| 4-1BB | 10.85 (1.95-70.9) | 6.01 (1.29-29.4) | .0038 |
| ICOS | 14.45 (1.41-68.4) | 16.85 (2.17-48.4) | .0854 |
| NK cells (CD3−, CD56+) | |||
| PD-1 | 3.69 (0.43-36.2) | 1.94 (0.098-22.5) | .0072 |
| TIM-3 | 4.11 (0-38.5) | 4.31 (2.13-18.4) | .6334 |
| LAG-3 | 1.58 (0-27.5) | 0.765 (0-29.8) | .0038 |
| OX-40 | 15.3 (1.23-77.5) | 4.1 (0.28-41.9) | <.0001 |
| CTLA-4 | 0.54 (0.016-18.6) | 0.25 (1-4.08) | .0298 |
| 4-1BB | 9.95 (1.24-47) | 5.005 (0.36-33) | .0023 |
| ICOS | 0.57 (0-8.35) | 0.52 (0.13-3.63) | .5216 |
Data are expressed as the median percentage of positivity and the range of each checkpoint is listed with P values via Mann-Whitney U tests, with significant differential expression set in bold.