ROTEM assay results using whole blood samples collected on day 140 after IO infusion of LVs to evaluate platelet FVIII function in HemA mice
| Mice . | Mean ± standard deviation of mean . | |||
|---|---|---|---|---|
| CT, s . | CFT, s . | α, degree . | MCF, mm . | |
| G-F8/N6K12RH-LV + drugs | 146 ± 4* | 104 ± 21 | 70 ± 4** | 66 ± 4 |
| G-F8/N6K12RH-LV | 388 ± 193 | 165 ± 123 | 62 ± 15* | 61 ± 7 |
| G-F8/N6-LV | 564 ± 367 | 261 ± 182 | 43 ± 13 | 62 ± 6 |
| Wild type | 164 ± 49* | 57 ± 10* | 79 ± 2** | 63 ± 1 |
| HemA | 956 ± 185* | 2370 ± 1150* | 4 ± 6*** | 26 ± 19* |
| Mice . | Mean ± standard deviation of mean . | |||
|---|---|---|---|---|
| CT, s . | CFT, s . | α, degree . | MCF, mm . | |
| G-F8/N6K12RH-LV + drugs | 146 ± 4* | 104 ± 21 | 70 ± 4** | 66 ± 4 |
| G-F8/N6K12RH-LV | 388 ± 193 | 165 ± 123 | 62 ± 15* | 61 ± 7 |
| G-F8/N6-LV | 564 ± 367 | 261 ± 182 | 43 ± 13 | 62 ± 6 |
| Wild type | 164 ± 49* | 57 ± 10* | 79 ± 2** | 63 ± 1 |
| HemA | 956 ± 185* | 2370 ± 1150* | 4 ± 6*** | 26 ± 19* |
Each assay was carried out for 1 h to obtain values of clotting time (CT), clot formation time (CFT), α angle, and maximum clot firmness (MCF). Statistical significance was analyzed based on relevance to G-F8/N6-LV–treated mouse group. Mice were treated with G-F8/N6K12RH-LV + drugs, G-F8/N6K12RH-LV, or G-F8/N6-LV. Untreated wild-type normal mice and HemA mice were used as positive and negative controls, respectively.
P < .05, **P < .01, ***P < .001.