@article{10.1182/blood-2008-03-143370,
    author = {Waterstrat, Amanda and Liang, Ying and Swiderski, Carol F. and Shelton, Brent J. and Van Zant, Gary},
    title = {Congenic interval of CD45/Ly-5 congenic mice contains multiple genes that may influence hematopoietic stem cell engraftment},
    journal = {Blood},
    volume = {115},
    number = {2},
    pages = {408-417},
    year = {2010},
    month = {01},
    abstract = {The B6.SJL-Ptprc(d)Pep3(b)/BoyJ (B6.SJL) congenic mouse strain, a valuable and widely used tool in murine bone marrow transplantation studies, has long been considered equivalent to the parental C57B/L6 (B6) strain with the exception of a small congenic interval on chromosome 1 harboring an alternative CD45/Ly-5 alloantigen (Ly-5.1). In this study we compared functional properties of stem and stromal cells between the strains, and delineated the boundary of the B6.SJL congenic interval. We identified a 25\% reduction in homing efficiency, 3.8-fold reduction in transplantable long-term hematopoietic stem cells (LT-HSCs), a 5-fold reduction in LT-HSCs capable of 24-hour homing, and a cell-intrinsic engraftment defect of 30\% to 50\% in B6.SJL-derived bone marrow cells relative to B6-derived cells. These functional differences were independent of stem cell number, cycling, or apoptosis. Genotypic analysis revealed a 42.1-mbp congenic interval in B6.SJL including 306 genes, and at least 124 genetic polymorphisms. Moreover, expression profiling revealed 288 genes differentially expressed between nonhematopoietic stromal cells of the 2 strains. These results indicate that polymorphisms between the B6 and SJL genotype within the B6.SJL congenic interval influence HSC engraftment and result in transcriptional variation within bone marrow stroma.},
    issn = {0006-4971},
    doi = {10.1182/blood-2008-03-143370},
    url = {https://doi.org/10.1182/blood-2008-03-143370},
    eprint = {https://ashpublications.org/blood/article-pdf/115/2/408/1489705/zh800210000408.pdf},
}